Key features and details
- Expression system: Baculovirus
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Product nameRecombinant human MKK6 protein
See all MKK6 proteins and peptides
Biological activityEnzyme reaction is conducted in a buffer containing 50 mM HEPES (pH7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, 100ng of inactive MAPK14 and 2 µM substrate (SER/THR 4) at room temperature for 1 hour. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
Protein lengthProtein fragment
Amino acids2 to 334
Our Abpromise guarantee covers the use of ab95252 in the following tested applications.
This product was previously labelled as MEK6
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0462% DTT, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Dual specificity mitogen activated protein kinase kinase 6
- Dual specificity mitogen-activated protein kinase kinase 6
- MAP kinase kinase 6
FunctionCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Tissue specificityIsoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
- Information by UniProt
ab95252 has not yet been referenced specifically in any publications.