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Recombinant Human MLF1 Interacting Protein/PBIP1 (denatured) (ab174458)

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SDS-PAGE - Recombinant Human MLF1 Interacting Protein/PBIP1  (denatured) (ab174458)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 80% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human MLF1 Interacting Protein/PBIP1 (denatured)
    • Purity

      > 80 % SDS-PAGE.

    • Expression system

      Escherichia coli
    • Accession

      Q71F23
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG
      • Predicted molecular weight

        34 kDa including tags
      • Amino acids

        147 to 418
      • Tags

        His tag N-Terminus
      • Additional sequence information

        NCBI Accession No. NP_078905.
    • Description

      Recombinant Human MLF1 Interacting Protein/PBIP1

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab174458 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 2.4% Urea, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • CENP U
      • CENP-50
      • CENP-U
      • CENP50
      • CENPU
      • CENPU_HUMAN
      • CENPU50
      • centromere protein of 50 kDa
      • Centromere protein U
      • FLJ23468
      • HHV8 LNAIP1
      • ICEN24
      • Interphase centromere complex protein 24
      • Kaposi Sarcoma Herpesvirus latent nuclear antigen interacting protein 1
      • KLIP-1
      • KLIP1
      • KSHV latent nuclear antigen interacting protein 1
      • KSHV latent nuclear antigen-interacting protein 1
      • KSHV LNAIP1
      • LNAIP 1
      • LNAIP-1
      • MLF1-interacting protein
      • MLF1IP
      • MLF1IP1
      • PBIP1
      • Polo-box-interacting protein 1
      see all
    • Function

      Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
    • Tissue specificity

      Expressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
    • Post-translational
      modifications

      Phosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
    • Cellular localization

      Cytoplasm. Nucleus. Chromosome > centromere > kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.
    • Target information above from: UniProt accession Q71F23 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human MLF1 Interacting Protein/PBIP1  (denatured) (ab174458)
      SDS-PAGE - Recombinant Human MLF1 Interacting Protein/PBIP1 (denatured) (ab174458)

      15% SDS-PAGE analysis of ab174458 (3μg).

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab174458? Please let us know so that we can cite the reference in this datasheet.

    ab174458 has not yet been referenced specifically in any publications.

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