Recombinant Human MLF1 Interacting Protein/PBIP1 (denatured) (ab174458)
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Overview
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Product nameRecombinant Human MLF1 Interacting Protein/PBIP1 (denatured)
See all MLF1 Interacting Protein/PBIP1 proteins and peptides -
Protein lengthProtein fragment
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DescriptionRecombinant Human MLF1 Interacting Protein/PBIP1
Description
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NatureRecombinant
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SourceEscherichia coli
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Amino Acid Sequence
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Accession
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SpeciesHuman
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SequenceMGSSHHHHHH SSGLVPRGSH MGSTRRKVKS AEKISTQRHE VIRTTASSEL SEKPAESVTS KKTGPLSAQP SVEKENLAIE SQSKTQKKGK ISHDKRKKSR SKAIGSDTSD IVHIWCPEGM KTSDIKELNI VLPEFEKTHL EHQQRIESKV CKAAIATFYV NVKEQFIKML KESQMLTNLK RKNAKMISDI EKKRQRMIEV QDELLRLEPQ LKQLQTKYDE LKERKSSLRN AAYFLSNLKQ LYQDYSDVQA QEPNVKETYD SSSLPALLFK ARTLLGAESH LRNINHQLEK LLDQG
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Molecular weight34 kDa including tags
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Amino acids147 to 418
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TagsHis tag N-Terminus
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Additional sequence informationNCBI Accession No. NP_078905.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab174458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Purity> 80 % SDS-PAGE.
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FormLiquid
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Information available upon request.
General Info
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Alternative names
- CENP U
- CENP-50
- CENP-U
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FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
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Tissue specificityExpressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
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Post-translational
modificationsPhosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1. -
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere > kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.
- Information by UniProt
Images
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SDS-PAGE - Recombinant Human MLF1 Interacting Protein/PBIP1 (denatured) (ab174458)15% SDS-PAGE analysis of ab174458 (3μg).
Datasheets and documents
References
ab174458 has not yet been referenced specifically in any publications.
