Recombinant human MLK2 protein (ab125562)

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Overview

Description

  • Nature

    Recombinant

  • Source

    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      Q02779

    • Species

      Human

    • Molecular weight

      76 kDa including tags

    • Amino acids

      1 to 446

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    The specific activity of ab125562 was determined to be 48 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity

    > 85 % Densitometry.
    Purity was determined to be >85% by densitometry.

  • Form

    Liquid

  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • M3K10_HUMAN
    • MAP3K 10
    • Map3k10
    • MEKK10
    • Mitogen activated protein kinase kinase kinase 10
    • Mitogen-activated protein kinase kinase kinase 10
    • Mixed lineage kinase 2
    • MKN28 derived nonreceptor type serine/threonine kinase
    • MKN28 kinase
    • MLK 2
    • MST
    • Protein kinase MST
    see all

  • Function

    Activates the JUN N-terminal pathway.

  • Tissue specificity

    Expressed in brain and skeletal muscle.

  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.

  • Post-translational
    modifications

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human MLK2 protein (ab125562)
    SDS-PAGE - Recombinant human MLK2 protein (ab125562)
    SDS-PAGE analysis of ab125562.
  • Functional Studies - Recombinant human MLK2 protein (ab125562)
    Functional Studies - Recombinant human MLK2 protein (ab125562)
    The specific activity of ab125562 was determined to be 48 nmol/min/mg by Kinase Assay.

References

ab125562 has not yet been referenced specifically in any publications.

Publishing research using ab125562? Please let us know so that we can cite the reference in this datasheet.

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