Overview

Description

  • Nature
    Recombinant
  • Source
    Baculovirus infected Sf9 cells
  • Amino Acid Sequence
    • Accession
      Q02779
    • Species
      Human
    • Molecular weight
      76 kDa including tags
    • Amino acids
      1 to 446

Associated products

Specifications

Our Abpromise guarantee covers the use of ab125562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity
    The specific activity of ab125562 was determined to be 48 nmol/min/mg.
  • Applications

    Western blot

    Functional Studies

    SDS-PAGE

  • Purity
    > 85 % Densitometry.
    Purity was determined to be >85% by densitometry.
  • Form
    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names
    • M3K10_HUMAN
    • MAP3K 10
    • Map3k10
    • MEKK10
    • Mitogen activated protein kinase kinase kinase 10
    • Mitogen-activated protein kinase kinase kinase 10
    • Mixed lineage kinase 2
    • MKN28 derived nonreceptor type serine/threonine kinase
    • MKN28 kinase
    • MLK 2
    • MST
    • Protein kinase MST
    see all
  • Function
    Activates the JUN N-terminal pathway.
  • Tissue specificity
    Expressed in brain and skeletal muscle.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications
    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant human MLK2 protein (ab125562)
    SDS-PAGE - Recombinant human MLK2 protein (ab125562)
    SDS-PAGE analysis of ab125562.
  • Functional Studies - Recombinant human MLK2 protein (ab125562)
    Functional Studies - Recombinant human MLK2 protein (ab125562)
    The specific activity of ab125562 was determined to be 48 nmol/min/mg by Kinase Assay.

References

ab125562 has not yet been referenced specifically in any publications.

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