Recombinant human MLK2 protein (ab125562)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human MLK2 protein -
Biological activity
The specific activity of ab125562 was determined to be 48 nmol/min/mg. -
Purity
> 85 % Densitometry.
Purity was determined to be >85% by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
76 kDa including tags -
Amino acids
1 to 446
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab125562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- M3K10_HUMAN
- MAP3K 10
- Map3k10
see all -
Function
Activates the JUN N-terminal pathway. -
Tissue specificity
Expressed in brain and skeletal muscle. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. - Information by UniProt
Images
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Functional Studies - Recombinant human MLK2 protein (ab125562)The specific activity of MLK2 (ab125562) was determined to be 50 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human MLK2 protein (ab125562)SDS PAGE analysis of ab125562 -
SDS-PAGE - Recombinant human MLK2 protein (ab125562)SDS PAGE analysis of ab125562 -
SDS-PAGE - Recombinant human MLK2 protein (ab125562)SDS-PAGE analysis of ab125562. -
Functional Studies - Recombinant human MLK2 protein (ab125562)The specific activity of ab125562 was determined to be 48 nmol/min/mg by Kinase Assay.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab125562 has not yet been referenced specifically in any publications.
