Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human MLK2 protein
Biological activityThe specific activity of ab125562 was determined to be 48 nmol/min/mg.
Purity> 85 % Densitometry.
Purity was determined to be >85% by densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight76 kDa including tags
Amino acids1 to 446
Our Abpromise guarantee covers the use of ab125562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- MAP3K 10
FunctionActivates the JUN N-terminal pathway.
Tissue specificityExpressed in brain and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125562 has not yet been referenced specifically in any publications.