Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human MLK4 protein (Active)
The specific activity of ab268798 was 22 nmol/min/mg in a kinase assay using MBP protein as substrate.
Purity> 80 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
SequenceSSPVHVAFERLELKELIGAGGFGQVYRATWQGQEVAVKAARQDPEQDAAA AAESVRREARLFAMLRHPNIIELRGVCLQQPHLCLVLEFARGGALNRALA AANAAPDPRAPGPRRARRIPPHVLVNWAVQIARGMLYLHEEAFVPILHRD LKSSNILLLEKIEHDDICNKTLKITDFGLAREWHRTTKMSTAGTYAWMAP EVIKSSLFSKGSDIWSYGVLLWELLTGEVPYRGIDGLAVAYGVAVNKLTL PIPSTCPEPFAKLMKECWQQDPHIRPSFALILEQLTAIEGAVMTEMPQES FHSMQDD
Molecular weight information61 kDa by SDS-PAGE
Amino acids114 to 420
TagsGST tag N-Terminus
Additional sequence informationNM_032435
Our Abpromise guarantee covers the use of ab268798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionActivates the JUN N-terminal pathway.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab268798 has not yet been referenced specifically in any publications.