Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human MMP1 protein (His tag)
See all MMP1 proteins and peptides
Purity> 95 % SDS-PAGE.
Purified by using conventional chromatography techniques.
Endotoxin level< 1.000 Eu/µg
Expression systemBaculovirus infected insect cells
Protein lengthFull length protein
SequenceHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQM QEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYR IENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGD HRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAA HELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPV QPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFI SVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSS FGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAH DFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCR KNLEHHHHHH
Predicted molecular weight53 kDa including tags
Amino acids18 to 469
TagsHis tag C-Terminus
Additional sequence informationThis product is the mature full length protein from aa 18 to 469. The signal peptide is not included.
- Anti-MMP1 antibody [EP1249Y] (ab134184)
- Anti-MMP1 antibody (ab137332)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-MMP1 antibody [EP1247Y] (Alexa Fluor® 647) (ab196905)
- Anti-MMP1 antibody [EP1247Y] (Alexa Fluor® 488) (ab197233)
- Anti-MMP1 antibody [EP1247Y] (PE) (ab209574)
- Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-MMP1 antibody [EP1247Y] (ab52631)
- Anti-6X His tag® antibody (ab9108)
Our Abpromise guarantee covers the use of ab222453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.05% Brij, 0.11% Calcium chloride, 30% Glycerol (glycerin, glycerine), 0.39% MES, 0.58% Sodium chloride
- 27 kDa interstitial collagenase
FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab222453 has not yet been referenced specifically in any publications.