The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specific Activity = =60mU/mg protein after trypsin activation. One unit is defined as the amount of enzyme that hydrolyzes 1µmol Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 per min. at 37°C, pH 7.5.
>90% by SDS-PAGE.
An aliquot of 5µg MMP14 proenzyme is mixed with 1µl trypsin solution in 100µl avtivation buffer and incubated for 12 min. at 25°C. Thereafter trypsin is inhibited by addition of 1µl aprotinin solution.
Activation buffer: 50mM TRIS-HCl, pH 7.5, 150mM NaCl, 5mM CaCl2. Store at -20°C.
Trypsin solution: 50µg TPCK-trypsin per ml activation buffer. Store in aliquots at -20°C.
Aprotinin solution: 1mg aprotinin per ml activation buffer. Store at -20°C.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Matrix metallopeptidase 14 (membrane inserted)
Matrix metalloproteinase 14
Membrane type 1 matrix metalloproteinase
Membrane type 1 metalloprotease
Membrane type matrix metalloproteinase 1
Membrane-type matrix metalloproteinase 1
Membrane-type-1 matrix metalloproteinase
MT MMP 1
Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7.
Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors.
Belongs to the peptidase M10A family. Contains 4 hemopexin-like domains.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
The precursor is cleaved by a furin endopeptidase.
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.