Product nameRecombinant human MMP2 protein
See all MMP2 proteins and peptides
Protein lengthFull length protein
SourceHEK 293 cells
Amino Acid Sequence
Molecular weight71 kDa including tags
Amino acids30 to 660
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab125181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Recombinant Human MMP-2 protein is a pro form. It needs to be activated with p-aminophenylmercuric acetate (APMA). Please contact our Scientific Support team for additional details.
The activity was measured by its ability to cleave the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2. The specific activity is > 1,000 pmoles/min/µg.
Purity> 95 % SDS-PAGE.
ab125181 is purified by proprietary chromatographic techniques.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 0.05% Brij, 0.32% Tris HCl, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- 72 kDa gelatinase
- 72kD type IV collagenase
- CLG 4
FunctionUbiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
Tissue specificityProduced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
Involvement in diseaseDefects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsPhosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
Cellular localizationSecreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
- Information by UniProt
Detection of gelatinolytic activity of ab125181 MMP2 protein.
10 ng of ab125181 in 25 µl of 25 mM Tris-glycine buffer (pH 6.8) was mixed with 2x SDS-sample buffer (without ß-mercaptoethanol) and incubated at 37°C for 10 minutes. Samples were loaded on a 10% acrylamide-Tris-glycine gel containing 0.1% gelatin and electrophoresed using Tris-glycine (pH 8.3) with 0.1% SDS. Electrophoresis was performed at 90V for 2 hours. The gel was washed 3 times in 40-50 ml of 2.5% Triton X-100 for 15 minutes. The gel was immersed in 40-50 ml of 50 mM Tris-glycine (pH 7.5), 100 mM NaCl, 4 mM CaCl2 and 0.02% Triton X-100 and incubated at 37°C for 16 hours. For detection of the gelatinolytic activity, the gel was stained with 0.05% Commassie Blue.
ab125181 produced a prominent gelatinolytic band corresponding to a molecular mass of ~ 68 kDa.
ab125181 has not yet been referenced specifically in any publications.