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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Recombinant Human MMP2 protein (His tag) (ab174022)

  • Datasheet
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SDS-PAGE - Recombinant human MMP2 protein (Active) (ab174022)

    Key features and details

    • Expression system: HEK 293 cells
    • Purity: > 92% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: Functional Studies, SDS-PAGE

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    Description

    • Product name

      Recombinant Human MMP2 protein (His tag)
      See all MMP2 proteins and peptides
    • Purity

      > 92 % SDS-PAGE.
      Less than 1.0 EU per µg by the LAL method.
    • Endotoxin level

      < 1.000 Eu/µg
    • Expression system

      HEK 293 cells
    • Accession

      P08253
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        APSPIIKFPG DVAPKTDKEL AVQYLNTFYG CPKESCNLFV LKDTLKKMQK FFGLPQTGDL DQNTIETMRK PRCGNPDVAN YNFFPRKPKW DKNQITYRII GYTPDLDPET VDDAFARAFQ VWSDVTPLRF SRIHDGEADI MINFGRWEHG DGYPFDGKDG LLAHAFAPGT GVGGDSHFDD DELWTLGEGQ VVRVKYGNAD GEYCKFPFLF NGKEYNSCTD TGRSDGFLWC STTYNFEKDG KYGFCPHEAL FTMGGNAEGQ PCKFPFRFQG TSYDSCTTEG RTDGYRWCGT TEDYDRDKKY GFCPETAMST VGGNSEGAPC VFPFTFLGNK YESCTSAGRS DGKMWCATTA NYDDDRKWGF CPDQGYSLFL VAAHEFGHAM GLEHSQDPGA LMAPIYTYTK NFRLSQDDIK GIQELYGASP DIDLGTGPTP TLGPVTPEIC KQDIVFDGIA QIRGEIFFFK DRFIWRTVTP RDKPMGPLLV ATFWPELPEK IDAVYEAPQE EKAVFFAGNE YWIYSASTLE RGYPKPLTSL GLPPDVQRVD AAFNWSKNKK TYIFAGDKFW RYNEVKKKMD PGFPKLIADA WNAIPDNLDA VVDLQGGGHS YFFKGAYYLK LENQSLKSVK FGSIKSDWLG C
      • Predicted molecular weight

        72 kDa including tags
      • Amino acids

        30 to 660
      • Tags

        His tag C-Terminus
      • Additional sequence information

        Full length mature protein, minus the signal peptide.

    Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-MMP2 antibody [4D3] (ab2462)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-MMP2 antibody [6E3F8] (ab86607)
      • Anti-6X His tag® antibody (ab9108)
      • Anti-MMP2 antibody [EPR1184] (ab92536)

    Specifications

    Our Abpromise guarantee covers the use of ab174022 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      Please avoid adding high concentration APMA solution (>20mM) directly into the reaction as it tends to precipitate. A pre-dilution step is highly recommended.

      Pre-activation is required for enzymatic assays. Details in the Reconstitution section.

      This product is stable after storage at:

      • -20°C to -70°C for 12 months in lyophilized state;
      • -70°C for 3 months under sterile conditions after reconstitution.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at Room Temperature. Upon delivery aliquot. Store at -20°C or -80°C. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).

      pH: 7.4
      Constituents: 0.87% Sodium chloride, 0.61% Tris, 5% Trehalose

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

    General Info

    • Alternative names

      • 72 kDa gelatinase
      • 72kD type IV collagenase
      • CLG 4
      • CLG 4A
      • CLG4
      • CLG4A
      • Collagenase Type 4 alpha
      • Collagenase type IV A
      • Gelatinase A
      • Gelatinase alpha
      • Gelatinase neutrophil
      • Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase
      • Matrix Metalloproteinase 2
      • Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)
      • Matrix metalloproteinase II
      • Matrix metalloproteinase-2
      • MMP 2
      • MMP II
      • MMP-2
      • MMP2
      • MMP2_HUMAN
      • MONA
      • Neutrophil gelatinase
      • PEX
      • TBE 1
      • TBE-1
      see all
    • Function

      Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-
      -Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro.
      PEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrinv/beta3 on the surface of blood vessels.
    • Tissue specificity

      Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate.
    • Involvement in disease

      Defects in MMP2 are the cause of Torg-Winchester syndrome (TWS) [MIM:259600]; also known as multicentric osteolysis nodulosis and arthropathy (MONA). TWS is an autosomal recessive osteolysis syndrome. It is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Torg-Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes.
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 3 fibronectin type-II domains.
      Contains 4 hemopexin-like domains.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications

      Phosphorylation on multiple sites modulates enzymatic activity. Phosphorylated by PKC in vitro.
      The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT-MMP3). Autocatalytic cleavage in the C-terminal produces the anti-angiogenic peptide, PEX. This processing appears to be facilitated by binding integrinv/beta3.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix. Membrane. Nucleus. Colocalizes with integrin alphaV/beta3 at the membrane surface in angiogenic blood vessels and melanomas. Found in mitochondria, along microfibrils, and in nuclei of cardiomyocytes.
    • Target information above from: UniProt accession P08253 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant human MMP2 protein (Active) (ab174022)
      SDS-PAGE - Recombinant human MMP2 protein (Active) (ab174022)

      Reduced ab174022 on SDS-PAGE, stained overnight with Coomassie Blue. The protein migrates as 66-71 kDa under reducing conditions.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (2)

    Publishing research using ab174022? Please let us know so that we can cite the reference in this datasheet.

    ab174022 has been referenced in 2 publications.

    • Liu SS  et al. A Novel Regulatory Axis, CHD1L-MicroRNA 486-Matrix Metalloproteinase 2, Controls Spermatogonial Stem Cell Properties. Mol Cell Biol 39:N/A (2019). PubMed: 30455250
    • Wen SL  et al. Collapsed Reticular Network and its Possible Mechanism during the Initiation and/or Progression of Hepatic Fibrosis. Sci Rep 6:35426 (2016). PubMed: 27739503

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