Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, Inhibition Assay
Product nameRecombinant human MMP3 protein
See all MMP3 proteins and peptides
ab134448 contains the catalytic portion of MMP3 and displays similar activity as the 28 kDa form generated from full-length MMP3. The specific activity of ab134448 was >1000 mU/mg when assayed with the synthetic substrate (7-methoxycoumarin-4yl) acetyl-Arg-Pro-Lys-Pro-ValGlu-Nva-Trp-Arg-Lys (Dnp) - NH2.
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
Predicted molecular weight22 kDa
Amino acids100 to 273
Our Abpromise guarantee covers the use of ab134448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 0.05% Sodium azide
Constituents: 0.1% Calcium chloride, 0.0001% Zinc chloride, 0.79% Tris HCl
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134448 has not yet been referenced specifically in any publications.