Recombinant Human MNAT1 protein (ab204195)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His-T7 tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human MNAT1 protein -
Purity
> 90 % SDS-PAGE.
ab204195 was refolded using unique “temperature shift inclusion body refolding” technology and chromatographically purified. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MASMTGGQQMGRGHHHHHHENLYFQGDDQGCPRCKTTKYRNPSLKLMVNV CGHTLCESCVDLLFVRGAGNCPECGTPLRKSNFRVQLFEDPTVDKEVEIR KKVLKIYNKREEDFPSLREYNDFLEEVEEIVFNLTNNVDLDNTKKKMEIY QKENKDVIQKNKLKLTREQEELEEALEVERQENEQRRLFIQKEEQLQQIL KRKNKQAFLDELESSDLPVALLLAQHKDRSTQLEMQLEKPKPVKPVTFST GIKMGQHISLAPIHKLEEALYEYQPLQIETYGPHVPELEMLGRLGYLNHV RAASPQDLAGGYTSSLACHRALQDAFSGLFWQPS -
Predicted molecular weight
39 kDa including tags -
Amino acids
2 to 309 -
Tags
His-T7 tag N-Terminus -
Additional sequence information
Constructed with codon optimization technology and expressed with a small T7-His-TEV cleavage site Tag (29aa) fusion at its N-terminal. This protein is expressed in E.coli as inclusion bodies.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab204195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.32% Tris HCl
Contains NaCl, KCl, EDTA, arginine, DTT and glycerol. Sterile-filtered
General Info
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Alternative names
- MNAT 1
- CAP35
- CDK activating kinase assembly factor MAT1
see all -
Function
Stabilizes the cyclin H-CDK7 complex to form a functional CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. -
Tissue specificity
Highest levels in colon and testis. Moderate levels are present thymus, prostate, ovary, and small intestine. The lowest levels are found in spleen and leukocytes. -
Sequence similarities
Contains 1 RING-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat. -
Cellular localization
Nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab204195 has not yet been referenced specifically in any publications.