Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Product nameRecombinant human MNK1 protein
See all MNK1 proteins and peptides
Biological activityThe specific activity of ab125635 was determined to be 16 - 20 nmol/min/mg.
Purity> 85 % Densitometry.
Purity was determined to be >85% by densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Predicted molecular weight92 kDa including tags
Amino acids1 to 465
Our Abpromise guarantee covers the use of ab125635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab204868 (Modified EIF2S peptide) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- MAP kinase interacting kinase 1
- MAP kinase interacting serine/threonine kinase 1
- MAP kinase signal integrating kinase 1
FunctionMay play a role in the response to environmental stress and cytokines. Appears to regulate transcription by phosphorylating EIF4E, thus increasing the affinity of this protein for the 7-methylguanosine-containing mRNA cap.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
modificationsDual phosphorylation of Thr-250 and Thr-255 activates the kinase. Phosphorylation of Thr-385 activates the kinase.
Cellular localizationCytoplasm and Cytoplasm. Nucleus.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab125635 has not yet been referenced specifically in any publications.