Recombinant Human Moesin protein (ab124306)

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SDS-PAGE - Recombinant Human Moesin protein (ab124306)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human Moesin protein
      See all Moesin proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Expression system

      Escherichia coli

    • Accession

      P26038

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Predicted molecular weight

        68 kDa

      • Amino acids

        1 to 577

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab124306 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

      Constituent: 0.48% HEPES

    General Info

    • Alternative names

      • Epididymis luminal protein 70
      • HEL70
      • Membrane organizing extension spike protein
      • Membrane-organizing extension spike protein
      • MOES_HUMAN
      • Moesin
      • Moesin/anaplastic lymphoma kinase fusion protein
      • Msn
      • MSN/ALK fusion
      see all

    • Function

      Probably involved in connections of major cytoskeletal structures to the plasma membrane.

    • Tissue specificity

      In all tissues and cultured cells studied.

    • Sequence similarities

      Contains 1 FERM domain.

    • Post-translational
      modifications

      Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures.

    • Cellular localization

      Cell membrane. Cytoplasm > cytoskeleton. Apical cell membrane. Cell projection > microvillus membrane. Phosphorylated form is enriched in microvilli-like structures at apical membrane (By similarity). Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Moesin protein (ab124306)
      SDS-PAGE - Recombinant Human Moesin protein (ab124306)
      SDS-PAGE using 1µg of ab124306.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab124306? Please let us know so that we can cite the reference in this datasheet.

    ab124306 has been referenced in 1 publication.

    • Chakraborty PK  et al. G protein-coupled receptor kinase GRK5 phosphorylates moesin and regulates metastasis in prostate cancer. Cancer Res 74:3489-500 (2014). PubMed: 24755472

    Customer reviews and Q&As

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    Question

    ab124306: more information on the reconsititution. Is the hepes to be added or is it already there. What does 0.48% HEPES means in the constituents section

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    Answer

    This is packed as 0.2 mg. This protein is lyophilized from 20mM HEPES, pH7.0.

    Resuspend with 200ul distilled water to produce 1 mg/ml concentration.

    Read More

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