Recombinant Human Mre11 protein (ab114277)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human Mre11 protein -
Expression system
Wheat germ -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MSTADALDDENTFKILVATDIHLGFMEKDAVRGNDTFVTLDEILRLAQEN EVDFILLGGDLFHENKPSRKTLHTCLELLRKYCMGDRPVQFEILSDQSVN FGFSKFPWVNYQDGNLNISIPVFSIHGNHDDPTGADALCALDILSCAGFV NHFGRSMSVEKIDISPVLLQKGRTKIALYGLGSIPDERLYRMFVNKKVTM LRPKED -
Predicted molecular weight
49 kDa including tags -
Amino acids
1 to 206
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Specifications
Our Abpromise guarantee covers the use of ab114277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
ELISA
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
This protein is best used within three months from the date of receipt.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.79% Tris HCl, 0.3% Glutathione
General Info
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Alternative names
- AT like disease
- Ataxia telangiectasia disorder like
- ATLD
see all -
Function
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. -
Involvement in disease
Defects in MRE11A are a cause of ataxia telangiectasia-like disorder (ATLD) [MIM:604391]. ATLD is a disease with the same clinical feature than ataxia-telangiectasia but with a somewhat milder clinical course. -
Sequence similarities
Belongs to the MRE11/RAD32 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Localizes to discrete nuclear foci after treatment with genotoxic agents. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Mre11 protein (ab114277)12.5% SDS-PAGE analysis of Mre11 protein (ab114277). Stained with Coomassie Blue.
Datasheets and documents
References
ab114277 has not yet been referenced specifically in any publications.
