Description

  • Product name

    Recombinant Human MRP8 protein (Tagged-His Tag)
    See all MRP8 proteins and peptides
  • Purity

    > 90 % SDS-PAGE.
    ab219249 was purified using conventional chromatography techniques.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      MGSSHHHHHH SSGLVPRGSH MGSHMLTELE KALNSIIDVY HKYSLIKGNF HAVYRDDLKK LLETECPQYI RKKGADVWFK ELDINTDGAV NFQEFLILVI KMGVAAHKKS HEESHKEEQK LISEEDL
    • Predicted molecular weight

      15 kDa including tags
    • Amino acids

      1 to 93
    • Tags

      His tag N-Terminus
    • Additional sequence information

      Myc-tag at C-terminus (NP_002955)

Specifications

Our Abpromise guarantee covers the use of ab219249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF
  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.0
    Constituents: 0.32% Tris HCl, 0.58% Sodium chloride, 10% Glycerol

General Info

  • Alternative names

    • 60B8Ag
    • AI323541
    • B8Ag
    • BEE11
    • CAGA
    • Calgranulin-A
    • Calprotectin L1L subunit
    • Calprotectin, included
    • CFAG
    • CGLA
    • Chemotactic cytokine CP-10
    • CP-10
    • Cystic fibrosis antigen
    • L1Ag
    • Leukocyte L1 complex light chain
    • MA387
    • MIF
    • Migration inhibitory factor-related protein 8
    • MRP-8
    • Myeloid-related protein 8
    • Neutrophil cytosolic 7 kDa protein
    • NIF
    • p8
    • Pro-inflammatory S100 cytokine
    • Protein S100-A8
    • S100 calcium binding protein A8
    • S100 calcium binding protein A8 (calgranulin A)
    • S100 calcium-binding protein A8
    • S100A8
    • S100A8/S100A9 complex, included
    • S10A8_HUMAN
    • Urinary stone protein band A
    see all
  • Function

    Calcium-binding protein. Has antimicrobial activity towards bacteria and fungi. Important for resistance to invasion by pathogenic bacteria. Up-regulates transcription of genes that are under the control of NF-kappa-B. Plays a role in the development of endotoxic shock in response to bacterial lipopolysaccharide (LPS) (By similarity). Promotes tubulin polymerization. Promotes phagocyte migration and infiltration of granulocytes at sites of wounding. Plays a role as pro-inflammatory mediator in acute and chronic inflammation and up-regulates the release of IL8 and cell-surface expression of ICAM1. Extracellular calprotectin binds to target cells and promotes apoptosis. Antimicrobial and proapoptotic activity is inhibited by zinc ions.
  • Tissue specificity

    Expressed by macrophages in chronic inflammations. Also expressed in epithelial cells constitutively or induced during dermatoses.
  • Sequence similarities

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Cellular localization

    Secreted. Cytoplasm. Cytoplasm > cytoskeleton. Cell membrane. Associates with tubulin filaments in activated monocytes. Targeted to the cell surface upon calcium influx. Released from blood leukocytes upon exposure to CSF2/GM-CSF, bacterial lipopolysaccharide (LPS) and during inflammatory processes. Serum levels are high in patients suffering from chronic inflammation.
  • Information by UniProt

Images

  • 15% SDS-PAGE analysis of ab219249 (3μg).

References

ab219249 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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