Recombinant human MST3 protein (ab51413)
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Overview
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Product nameRecombinant human MST3 protein
See all MST3 proteins and peptides -
Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceBaculovirus infected Sf9 cells
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Amino Acid Sequence
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SpeciesHuman
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Amino acids1 to 311
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab51413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Biological activitySpecific activity 265 nmol/min/mg.
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Applications
Functional Studies
SDS-PAGE
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FormLiquid
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Additional notes
ab204875 (PKC epsilon peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.87% Sodium chloride, 25% Glycerol, 0.00292% EDTA, 0.79% Tris HCl, 0.00385% DTT, 0.00174% PMSF, 0.0038% EGTAThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- epididymis secretory protein Li 95
- HEL-S-95
- Mammalian STE20 like protein kinase 3
see all -
FunctionSerine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. Regulates cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve.
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Tissue specificityIsoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex.
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Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsProteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis.
Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues. -
Cellular localizationCytoplasm. Nucleus. Membrane. The truncated form (MST3/N) translocates to the nucleus. Co-localizes with STK38L in the membrane.
- Information by UniProt
Images
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Functional Studies - Recombinant human MST3 protein (ab51413)Kinase activity assay - specific activity 265 nmol/min/mg. -
SDS-PAGE - Recombinant human MST3 protein (ab51413)Molecular weight 63 kDa
Datasheets and documents
References
ab51413 has not yet been referenced specifically in any publications.
