Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Product nameRecombinant human MST4 protein
Purity> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
Our Abpromise guarantee covers the use of ab60341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- EC 220.127.116.11
- Mammalian Ste20 like protein kinase 4
FunctionMediator of cell growth. Modulates apoptosis.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Golgi apparatus membrane. Localizes to the Golgi apparatus.
- Information by UniProt
The specific activity of MST4 (ab60341) was determined to be 23 nmol/min/mg as per activity assay protocol
SDS PAGE analysis of ab60341
Sample Kinase Activity Plot.
Anti-MST4 antibody [EP1864Y] (ab52491) at 1/20000 dilution +
Recombinant human MST4 protein (ab60341) at 0.01 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Exposure time: 20 seconds
Ab52491 recognizes the full length tagged recombinant MST4 protein (ab60341) which has an expected molecular weight of 72 kDa.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab60341 has not yet been referenced specifically in any publications.