Recombinant Human MTHFD1 protein (ab116852)

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SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: ELISA, SDS-PAGE, WB

    Description

    • Product name

      Recombinant Human MTHFD1 protein

    • Expression system

      Wheat germ

    • Accession

      P11586

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSN LYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQ LPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPK GCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTA HLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGR KVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKP GKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGE TKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAH LYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAIT AANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRL GIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAP TEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPV SAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSII ADQIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVAT VRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGI PVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQ AVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAE VYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFL YPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

      • Predicted molecular weight

        129 kDa including tags

      • Amino acids

        1 to 935

    Specifications

    Our Abpromise guarantee covers the use of ab116852 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      SDS-PAGE

      Western blot

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase
      • C 1 tetrahydrofolate synthase cytoplasmic
      • C1 tetrahydrofolate synthase cytoplasmic
      • C1 THF synthase
      • C1-THF synthase
      • C1TC
      • C1TC_HUMAN
      • Cytoplasmic C 1 tetrahydrofolate synthase
      • Formyltetrahydrofolate synthetase
      • Methenyltetrahydrofolate cyclohydrolase
      • Methylenetetrahydrofolate dehydrogenase
      • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase
      • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase
      • MTHFC
      • MTHFD
      • MTHFD1
      • NADP dependent cyclohydrolase formyltetrahydrofolate synthetase
      • OTTHUMP00000180657
      see all

    • Tissue specificity

      Ubiquitous.

    • Pathway

      One-carbon metabolism; tetrahydrofolate interconversion.

    • Involvement in disease

      Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
      Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500].

    • Sequence similarities

      In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
      In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

    • Domain

      This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)
      SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)
      12.5% SDS-PAGE showing ab116852 at approximately 128.59kDa; stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab116852? Please let us know so that we can cite the reference in this datasheet.

    ab116852 has not yet been referenced specifically in any publications.

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