Recombinant Human MTHFD1 protein (ab116852)

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Description

  • Product name

    Recombinant Human MTHFD1 protein

  • Expression system

    Wheat germ

  • Accession

    P11586

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MAPAEILNGKEISAQIRARLKNQVTQLKEQVPGFTPRLAILQVGNRDDSN LYINVKLKAAEEIGIKATHIKLPRTTTESEVMKYITSLNEDSTVHGFLVQ LPLDSENSINTEEVINAIAPEKDVDGLTSINAGRLARGDLNDCFIPCTPK GCLELIKETGVPIAGRHAVVVGRSKIVGAPMHDLLLWNNATVTTCHSKTA HLDEEVNKGDILVVATGQPEMVKGEWIKPGAIVIDCGINYVPDDKKPNGR KVVGDVAYDEAKERASFITPVPGGVGPMTVAMLMQSTVESAKRFLEKFKP GKWMIQYNNLNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGE TKAKVLLSALERLKHRPDGKYVVVTGITPTPLGEGKSTTTIGLVQALGAH LYQNVFACVRQPSQGPTFGIKGGAAGGGYSQVIPMEEFNLHLTGDIHAIT AANNLVAAAIDARIFHELTQTDKALFNRLVPSVNGVRRFSDIQIRRLKRL GIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQAP TEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPV SAEDLGVSGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSII ADQIALKLVGPEGFVVTEAGFGADIGMEKFFNIKCRYSGLCPHVVVLVAT VRALKMHGGGPTVTAGLPLPKAYIQENLELVEKGFSNLKKQIENARMFGI PVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHWAEGGKGALALAQ AVQRAAQAPSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQHKAE VYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFL YPLVGTMSTMPGLPTRPCFYDIDLDPETEQVNGLF

    • Predicted molecular weight

      129 kDa including tags

    • Amino acids

      1 to 935

Specifications

Our Abpromise guarantee covers the use of ab116852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form

    Liquid

  • Additional notes

    This protein is best used within three months from the date of receipt.

     

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.3% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • 5,10 methylenetetrahydrofolate dehydrogenase 5,10 methylenetetrahydrofolate cyclohydrolase,10 formyltetrahydrofolate synthetase
    • C 1 tetrahydrofolate synthase cytoplasmic
    • C1 tetrahydrofolate synthase cytoplasmic
    • C1 THF synthase
    • C1-THF synthase
    • C1TC
    • C1TC_HUMAN
    • Cytoplasmic C 1 tetrahydrofolate synthase
    • Formyltetrahydrofolate synthetase
    • Methenyltetrahydrofolate cyclohydrolase
    • Methylenetetrahydrofolate dehydrogenase
    • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase
    • Methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 methenyltetrahydrofolate cyclohydrolase formyltetrahydrofolate synthetase
    • MTHFC
    • MTHFD
    • MTHFD1
    • NADP dependent cyclohydrolase formyltetrahydrofolate synthetase
    • OTTHUMP00000180657
    see all

  • Tissue specificity

    Ubiquitous.

  • Pathway

    One-carbon metabolism; tetrahydrofolate interconversion.

  • Involvement in disease

    Defects in MTHFD1 may be a cause of susceptibility to folate-sensitive neural tube defects (folate-sensitive NTD) [MIM:601634]. The most common NTDs are open spina bifida (myelomeningocele) and anencephaly. Genetic defects in MTHFD1 may affect the risk of spina bifida via the maternal rather than the embryonic genotype.
    Genetic variation in MTHFD1 could be associated with susceptibility to colorectal cancer (CRC) [MIM:114500].

  • Sequence similarities

    In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
    In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

  • Domain

    This trifunctional enzyme consists of two major domains: an N-terminal part containing the methylene-THF dehydrogenase and cyclohydrolase activities and a larger C-terminal part containing formyl-THF synthetase activity.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)
    SDS-PAGE - Recombinant Human MTHFD1 protein (ab116852)
    12.5% SDS-PAGE showing ab116852 at approximately 128.59kDa; stained with Coomassie Blue.

References

ab116852 has not yet been referenced specifically in any publications.

Publishing research using ab116852? Please let us know so that we can cite the reference in this datasheet.

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