Recombinant Human MURF1 protein (ab112428)
Key features and details
- Expression system: Wheat germ
- Suitable for: SDS-PAGE, ELISA, WB
Description
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Product name
Recombinant Human MURF1 protein -
Expression system
Wheat germ -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
DKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFE NMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGH -
Predicted molecular weight
37 kDa including tags -
Amino acids
254 to 352
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Specifications
Our Abpromise guarantee covers the use of ab112428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
ELISA
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- E3 ubiquitin-protein ligase TRIM63
- FLJ32380
- IRF
see all -
Function
E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. -
Tissue specificity
Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger. -
Developmental stage
Expressed throughout all developmental stages. -
Domain
The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity. -
Cellular localization
Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human MURF1 protein (ab112428)ab112428 analysed on a 12.5% SDS-PAGE gel stained with Coomassie Blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab112428 has not yet been referenced specifically in any publications.
