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    recombinant-human-myelin-basic-protein-tagged-ab43614.pdf

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Neuroscience Cell Adhesion Proteins Membrane Proteins
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Recombinant Human Myelin Basic Protein (Tagged) (ab43614)

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SDS-PAGE - Recombinant Human Myelin Basic Protein (Tagged) (ab43614)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 65% Densitometry
    • Tags: GST tag N-Terminus
    • Suitable for: Functional Studies, SDS-PAGE

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    Description

    • Product name

      Recombinant Human Myelin Basic Protein (Tagged)
      See all Myelin Basic Protein proteins and peptides
    • Purity

      > 65 % Densitometry.

    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSPEFM ASQKRPSQRHGSKYLATAST MDHARHGFLP RHRDTGILDS IGRFFGGDRG APKRGSGKDS HHPARTAHYGSLPQKSHGRT QDENPVVHFF KNIVTPRTPP PSQGKGRGLS LSRFSWGAEG QRPGFGYGGRASDYKSAHKG FKGVDAQGTL SKIFKLGGRD SRSGSPMARR
      • Predicted molecular weight

        36 kDa including tags
      • Tags

        GST tag N-Terminus
      • Additional sequence information

        GST-tag ID: P08515 (226aa), Thrombin site: 221-226aa, 3-AA Linker: 227-229aa, MBP protein: 230-400aa (NM_001025090).

    Associated products

    • Recombinant Protein

      • Recombinant human Aurora B protein (ab51435)
      • Recombinant human CaMKK protein (ab55692)
      • Recombinant human MAP3K8/COT protein (ab55700)
      • Recombinant human MAP4K6 protein (ab55712)
      • Recombinant human MEK2 protein (ab60013)
      • Recombinant human ITK/EMT protein (ab60300)
      • Recombinant human MST4 protein (ab60341)
      • Recombinant human TXK/RLK protein (ab60900)
      • Recombinant mouse PAK3 protein (ab61414)
      • Recombinant human TGF beta Receptor II protein (ab63173)
      • Recombinant human IPL-1/STK13/Aurora C protein (ab63177)
    • Related Products

      • Anti-GST antibody [EPR4236] (ab111947)
      • Anti-Myelin Basic Protein antibody [22] (ab11223)
      • Anti-Myelin Basic Protein antibody (ab28541)
      • Anti-Myelin Basic Protein antibody [12] (ab7349)
      • Anti-GST antibody (ab9085)

    Specifications

    Our Abpromise guarantee covers the use of ab43614 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

    • Form

      Liquid
    • Additional notes

      ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for the following active protein Kinases:

       

      ab51435 (Active human Aurora B full length protein)
      ab55692 (Active human CaMKK full length protein)
      ab55700 (Active human MAP3K8 protein fragment)

      ab55712 (Active human MAP4K6 protein fragment)

      ab55721 (Active Raf1 (mutated F341 Q) protein fragment)
      ab60013 (Active human MEK2 full length protein)

      ab60300 (Active ITK protein fragment)
      ab60325 (Active human ASK1 protein fragment)
      ab60341 (Active human MST4 full length protein)
      ab60900 (Active TXK protein fragment)
      ab61414 (Active mouse PAK3 full length protein)
      ab63173 (Active TGF beta Receptor II protein fragment)
      ab63177 (Active human IPL-1/STK13/Aurora C full length protein)

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 7.50
      Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride, 0.31% Glutathione

    General Info

    • Alternative names

      • GDB
      • Golli MBP
      • Golli MBP; myelin basic protein
      • Hemopoietic MBP
      • HMBPR
      • HUGO
      • MBP
      • MBP_CAVPO
      • MBP_HUMAN
      • MGC99675
      • MLD
      • Myelin A1 protein
      • Myelin A1 Protein, basic
      • Myelin basic protein
      • Myelin Deficient
      • Myelin membrane encephalitogenic protein
      • OTTHUMP00000163776
      • OTTHUMP00000174387
      • OTTHUMP00000174388
      • SHI
      • Shiverer
      • SP
      see all
    • Function

      The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
    • Tissue specificity

      MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
    • Involvement in disease

      Note=The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
    • Sequence similarities

      Belongs to the myelin basic protein family.
    • Developmental stage

      Expression begins abruptly in 14-16 week old fetuses. Even smaller isoforms seem to be produced during embryogenesis; some of these persisting in the adult. Isoform 4 expression is more evident at 16 weeks and its relative proportion declines thereafter.
    • Post-translational
      modifications

      Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic.
      The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6).
      Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
    • Cellular localization

      Myelin membrane. Cytoplasmic side of myelin.
    • Target information above from: UniProt accession P02686 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human Myelin Basic Protein (Tagged) (ab43614)
      SDS-PAGE - Recombinant Human Myelin Basic Protein (Tagged) (ab43614)

      SDS-PAGE analysis of ab43614.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab43614? Please let us know so that we can cite the reference in this datasheet.

    ab43614 has not yet been referenced specifically in any publications.

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