Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 75% Densitometry
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: WB, Functional Studies
Product nameRecombinant human MYLK2 protein
Specific activity: 270nmol/min/mg.
Purity> 75 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
TagsHis tag N-Terminus
Our Abpromise guarantee covers the use of ab84800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab60014 (Mouse MYL9 full length protein) can be utilized as a substrate for assessing Kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00308% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- MLCK, skeletal/cardiac
FunctionImplicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.
Tissue specificityHeart and skeletal muscles. Increased expression in the apical tissue compared to the interventricular septal tissue.
Involvement in diseaseDefects in MYLK2 are a cause of familial hypertrophic cardiomyopathy (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Cellular localizationCytoplasm. Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84800 has not yet been referenced specifically in any publications.