Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Product nameRecombinant Human MyoD1 protein
See all MyoD1 proteins and peptides
Purity> 90 % SDS-PAGE.
ab134857 was expressed in E.coli, refolded, chromatographically purified and sterile filtered.
Expression systemEscherichia coli
Protein lengthFull length protein
Sequence29aa_Tag_ELLSPPLRDVDLTAPDGSLCSFATTDDFYDDPCFDSPDLRF FEDLDPRLMHVGALLKPEEHSHFPAAVHPAPGAREDEHVRAPSGHHQAGR CLLWACKACKRKTTNADRRKAATMRERRRLSKVNEAFETLKRCTSSNPNQ RLPKVEILRNAIRYIEGLQALLRDQDAAPPGAAAAFYAPGPLPPGRGGEH YSGDSDASSPRSNCSDGMMDYSGPPSGARRRNCYEGAYYNEAPSEPRPGK SAAVSSLDCLSSIVERISTESPAAPALLLADVPSESPPRRQEAAAPSEGE SSGDPTQSPDAAPQCPAGANPNPIYQVLLEESGGGGSPGRRRRRRRRRRR
Predicted molecular weight37 kDa including tags
Amino acids2 to 320
Our Abpromise guarantee covers the use of ab134857 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituent: 0.32% Tris HCl
Buffer contains a proprietary formulation of NaCl, KCl, EDTA, Arginine, DTT and Glycerol.
- Class C basic helix-loop-helix protein 1
- MYF 3
FunctionInvolved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Activates muscle-specific promoters. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
modificationsAcetylated by a complex containing EP300 and PCAF. The acetylation is essential to activate target genes. Conversely, its deacetylation by SIRT1 inhibits its function.
Ubiquitinated on the N-terminus; which is required for proteasomal degradation.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab134857 has been referenced in 1 publication.
- Patel S et al. Inducing Stem Cell Myogenesis Using NanoScript. ACS Nano 9:6909-17 (2015). PubMed: 26108385