Overview

Description

  • Nature

    Recombinant
  • Source

    Wheat germ
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVLAKKGTSKTPVPEKVP PPKPATPDFRSVLGGKKKLPAENGSSSAETLNAKAVESSKPLSNAQPSGP LKPVGNAKPAETLKPMGNAKPAETLKPMGNAKPDENLKSASKEELKKDVK NDVNCKRGHAGTTDNEKRSESQGTAPAFKQKLQDVHVAEGKKLLLQCQVS SDPPATIIWTLNGKTLKTTKFIILSQEGSLCSVSIEKALPEDRGLYKCVA KNDAGQAECSCQVTVDDAPASENTKAPEMKSRRPKSSLPPVLGTESDATV KKKPAPKTPPKAAMPPQIIQFPEDQKVRAGESVELFGKVTGTQPITCTWM KFRKQIQESEHMKVENSENGSKLTILAARQEHCGCYTLLVENKLGSRQAQ VNLTVVDKPDPPAGTPCASDIRSSSLTLSWYGSSYDGGSAVQSYSIEIWD SANKTWKELATCRSTSFNVQDLLPDHEYKFRVRAINVYGTSEPSQESELT TVGEKPEEPKDEVEVSDDDEKEPEVDYRTVTINTEQKVSDFYDIEERLGS GKFGQVFRLVEKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLHHPKLV QCVDAFEEKANIVMVLEIVSGGELFERIIDEDFELTERECIKYMRQISEG VEYIHKQGIVHLDLKPENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLF GTPEFVAPEVINYEPIGYATDMWSIGVICYILVSGLSPFMGDNDNETLAN VTSATWDFDDEAFDEISDDAKDFISNLLKKDMKNRLDCTQCLQHPWLMKD TKNMEAKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMAMISGLSGRKS STGSPTSPLNAEKLESEEDVSQAFLEAVAEEKPHVKPYFSKTIRDLEVVE GSAARFDCKIEGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSLIISDV CGDDDAKYTCKAVNSLGEATCTAELIVETMEEGEGEGEEEEE
    • Molecular weight

      137 kDa including tags
    • Amino acids

      1 to 992

Specifications

Our Abpromise guarantee covers the use of ab152552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    SDS-PAGE

    Western blot

  • Form

    Liquid
  • Additional notes

    Protein concentration is above or equal to 0.05 µg/µl.

    Previously labelled as Myosin light chain kinase.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 8.00
    Constituents: 0.31% Glutathione, 0.79% Tris HCl

General Info

  • Alternative names

    • deglutamylated form
    • DKFZp686I10125
    • EC 2.7.11.18
    • FLJ12216
    • Kinase related protein
    • Kinase-related protein
    • KRP
    • MLCK
    • MLCK1
    • MLCK108
    • MLCK210
    • MSTP083
    • MYLK
    • MYLK_HUMAN
    • MYLK1
    • Myosin light chain kinase
    • Myosin light polypeptide kinase
    • OTTHUMP00000180642
    • OTTHUMP00000180643
    • smMLCK
    • smooth muscle
    • Smooth muscle myosin light chain kinase
    • Telokin
    see all
  • Function

    Calcium/calmodulin-dependent enzyme implicated in smooth muscle contraction via phosphorylation of myosin light chains (MLC). Also regulates actin-myosin interaction through a non-kinase activty (By similarity). Implicated in the regulation of endothelial as well as vascular permeability. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. Critical participant in signaling sequences that result in fibroblast apoptosis.
  • Tissue specificity

    Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. Telokin has been found in a wide variety of adult and fetal tissues.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family.
    Contains 1 fibronectin type-III domain.
    Contains 9 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    MLCK is probably down-regulated by phosphorylation.
    The C-terminus is deglutamylated by AGTPBP1/ CCP1, AGBL1/CCP4 and AGBL4/CCP6, leading to the formation of Myosin light chain kinase, smooth muscle, deglutamylated form. The consequences of C-terminal deglutamylation are unknown.
  • Information by UniProt

Images

  • 12.5% SDS-PAGE analysis of ab152552 stained with Coomassie Blue.

References

ab152552 has not yet been referenced specifically in any publications.

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