Recombinant Human Nanog protein (ab50053)

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Sandwich ELISA - Recombinant Human Nanog protein (ab50053)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 0.100 Eu/µg
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Human Nanog protein
      See all Nanog proteins and peptides

    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 0.100 Eu/µg

    • Expression system

      Escherichia coli

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV

      • Predicted molecular weight

        35 kDa

      • Amino acids

        1 to 304

    Specifications

    Our Abpromise guarantee covers the use of ab50053 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized

    • Additional notes

      Endotoxin level is less than 0.1 ng per µg (1EU/µg).
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    • Reconstitution
      Reconstitute to 1mg/ml using 10mM Acetic acid.

    General Info

    • Alternative names

      • Embryonic stem cell specific homeobox protein (Nanog)
      • ENK
      • FLJ12581
      • hNanog
      • Homeobox protein NANOG
      • Homeobox transcription factor Nanog
      • homeobox transcription factor Nanog-delta 48
      • NANOG
      • Nanog homeobox
      • NANOG_HUMAN
      see all

    • Function

      Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

    • Tissue specificity

      Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

    • Sequence similarities

      Belongs to the Nanog homeobox family.
      Contains 1 homeobox DNA-binding domain.

    • Developmental stage

      Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab50053? Please let us know so that we can cite the reference in this datasheet.

    ab50053 has been referenced in 2 publications.

    • Chikkaveeraiah BV  et al. Ultrasensitive nanostructured immunosensor for stem and carcinoma cell pluripotency gatekeeper protein NANOG. Nanomedicine (Lond) 7:957-65 (2012). PubMed: 22394186
    • Yang WC  et al. Cell-free production of transducible transcription factors for nuclear reprogramming. Biotechnol Bioeng 104:1047-58 (2009). ICC/IF, ELISA . PubMed: 19718703

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Question

    Thank you for the information. The question was-which cDNA-NCBI# corresponds to this protein, as there are several sequences.

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    Answer

    Thank you for your patience.

    I have obtained the following additional information from the lab:

    1) The purification details are proprietary, but 3 ion-exchange columns and no antibody or affinity columns were used.

    2) The information about the cDNA used and its design is proprietary.

    3) The protein sequence corresponds to the Expasy/SwissProt accession number: Q9H9S0 (http://pir.uniprot.org/uniprot/Q9H9S0)

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Question

    Please tell me -how your protein was isolated and what cDNA#-it was designed. I need it for the paper.

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    Answer

    Thank you for contacting us.

    The current information I have received from the supplying lab is as follows:

    Unfortunately, the purification details are proprietary. There were no tags used in the expression of this protein.

    I have contacted them again to see if they could provide some additional information, but I am not completely certain about that - but I had to try. I will be in touch, when I hear back from the lab. Thank you for your patience.

    In the meantime, please do not hesitate to contact us if you need any more advice or information.

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