Recombinant Human Nanog protein (ab50053)

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Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV
    • Molecular weight
      35 kDa
    • Amino acids
      1 to 304

Specifications

Our Abpromise guarantee covers the use of ab50053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 95 % SDS-PAGE.

  • Form
    Lyophilised
  • Additional notes
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    Preservative: None
    Endotoxin level is less than 0.1 ng per µg (1EU/µg).

  • Reconstitution
    Reconstitute to 1mg/ml using 10mM Acetic acid.

General Info

  • Alternative names
    • Embryonic stem cell specific homeobox protein (Nanog)
    • ENK
    • FLJ12581
    • hNanog
    • Homeobox protein NANOG
    • Homeobox transcription factor Nanog
    • homeobox transcription factor Nanog-delta 48
    • NANOG
    • Nanog homeobox
    • NANOG_HUMAN
    see all
  • Function
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificity
    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similarities
    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localization
    Nucleus.
  • Information by UniProt

Images

References

This product has been referenced in:
  • Yang WC  et al. Cell-free production of transducible transcription factors for nuclear reprogramming. Biotechnol Bioeng 104:1047-58 (2009). ICC/IF, ELISA . Read more (PubMed: 19718703) »
See 1 Publication for this product

Publishing research using ab50053? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Question

Thank you for the information. The question was-which cDNA-NCBI# corresponds to this protein, as there are several sequences.

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Answer

Thank you for your patience.

I have obtained the following additional information from the lab:

1) The purification details are proprietary, but 3 ion-exchange columns and no antibody or affinity columns were used.

2) The information about the cDNA used and its design is proprietary.

3) The protein sequence corresponds to the Expasy/SwissProt accession number: Q9H9S0 (http://pir.uniprot.org/uniprot/Q9H9S0)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Please tell me -how your protein was isolated and what cDNA#-it was designed. I need it for the paper.

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Answer

Thank you for contacting us.

The current information I have received from the supplying lab is as follows:

Unfortunately, the purification details are proprietary. There were no tags used in the expression of this protein.

I have contacted them again to see if they could provide some additional information, but I am not completely certain about that - but I had to try. I will be in touch, when I hear back from the lab. Thank you for your patience.

In the meantime, please do not hesitate to contact us if you need any more advice or information.

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