For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    recombinant-human-nanog-protein-ab50053.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Stem Cells Embryonic Stem Cells Intracellular
Share by email

Recombinant Human Nanog protein (ab50053)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Sandwich ELISA - Recombinant Human Nanog protein (ab50053)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 0.100 Eu/µg
    • Suitable for: SDS-PAGE

    You may also be interested in

    ELISA
    Product image
    Human Nanog ELISA Kit (ab236720)
    Pair
    Product image
    Human Nanog Antibody Pair - BSA and Azide free (ab244150)

    View more associated products

    Description

    • Product name

      Recombinant Human Nanog protein
      See all Nanog proteins and peptides
    • Purity

      > 95 % SDS-PAGE.

    • Endotoxin level

      < 0.100 Eu/µg
    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        SVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDV
      • Predicted molecular weight

        35 kDa
      • Amino acids

        1 to 304

    Associated products

    • Related Products

      • Anti-Nanog antibody (ab14959)
      • Anti-Nanog antibody (ab21624)
      • Anti-Nanog antibody (ab77095)

    Specifications

    Our Abpromise guarantee covers the use of ab50053 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Additional notes

      Endotoxin level is less than 0.1 ng per µg (1EU/µg).
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

    • Reconstitution
      Reconstitute to 1mg/ml using 10mM Acetic acid.

    General Info

    • Alternative names

      • Embryonic stem cell specific homeobox protein (Nanog)
      • ENK
      • FLJ12581
      • hNanog
      • Homeobox protein NANOG
      • Homeobox transcription factor Nanog
      • homeobox transcription factor Nanog-delta 48
      • NANOG
      • Nanog homeobox
      • NANOG_HUMAN
      see all
    • Function

      Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
    • Tissue specificity

      Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
    • Sequence similarities

      Belongs to the Nanog homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Developmental stage

      Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9H9S0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Sandwich ELISA - Recombinant Human Nanog protein (ab50053)
      Sandwich ELISA - Recombinant Human Nanog protein (ab50053)
      Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (2)

    Publishing research using ab50053? Please let us know so that we can cite the reference in this datasheet.

    ab50053 has been referenced in 2 publications.

    • Chikkaveeraiah BV  et al. Ultrasensitive nanostructured immunosensor for stem and carcinoma cell pluripotency gatekeeper protein NANOG. Nanomedicine (Lond) 7:957-65 (2012). PubMed: 22394186
    • Yang WC  et al. Cell-free production of transducible transcription factors for nuclear reprogramming. Biotechnol Bioeng 104:1047-58 (2009). ICC/IF, ELISA . PubMed: 19718703

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    Thank you for the information. The question was-which cDNA-NCBI# corresponds to this protein, as there are several sequences.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 21 2012

    Answer

    Thank you for your patience.

    I have obtained the following additional information from the lab:

    1) The purification details are proprietary, but 3 ion-exchange columns and no antibody or affinity columns were used.

    2) The information about the cDNA used and its design is proprietary.

    3) The protein sequence corresponds to the Expasy/SwissProt accession number: Q9H9S0 (http://pir.uniprot.org/uniprot/Q9H9S0)

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 21 2012

    Question

    Please tell me -how your protein was isolated and what cDNA#-it was designed. I need it for the paper.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 20 2012

    Answer

    Thank you for contacting us.

    The current information I have received from the supplying lab is as follows:

    Unfortunately, the purification details are proprietary. There were no tags used in the expression of this protein.

    I have contacted them again to see if they could provide some additional information, but I am not completely certain about that - but I had to try. I will be in touch, when I hear back from the lab. Thank you for your patience.

    In the meantime, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Mar 20 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.