Recombinant Human Nck protein (ab172816)

Our Abpromise guarantee covers the use of ab172816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Applications

SDS-PAGE

HPLC
Endotoxin level

< 1.000 Eu/µg
Purity

>95% by SDS-PAGE .
Form

Lyophilised
Additional notes

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Concentration information loading...

Stability and Storage

Shipped at Room Temperature. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Please see notes section.

pH: 8.00
Constituents: 0.24% Tris, 0.29% Sodium chloride

Supplied as a 0.2 µM filtered solution.
Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Alternative names
- Cytoplasmic protein NCK1
- Melanoma Nck protein
- MGC12668
- NCK
- NCK 1
- NCK adaptor protein 1
- NCK alpha
- NCK tyrosine kinase
- Nck-1
- NCK1
- NCK1_HUMAN
- NCKalpha
- Non catalytic region of tyrosine kinase
- SH2/SH3 adaptor protein NCK alpha
- SH2/SH3 adaptor protein NCK-alpha
see all
Function

Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Sequence similarities

Contains 1 SH2 domain.
Contains 3 SH3 domains.
Post-translational
modifications

Phosphorylated on Ser and Tyr residues.
Cellular localization

Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
Target information above from: UniProt accession P16333 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt

ab172816 has not yet been referenced specifically in any publications.

Publishing research using ab172816? Please let us know so that we can cite the reference in this datasheet.

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