The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
54 kDa VacA-interacting protein
54 kDa vimentin-interacting protein
90 kDa SH3 protein interacting with Nck
dia interacting protein
Dia-interacting protein 1
Diaphanous protein-interacting protein
NCK interacting protein with SH3 domain
NCK-interacting protein with SH3 domain
SH3 adapter protein SPIN90
SH3 protein interacting with Nck 90 kDa
WASP-interacting SH3-domain protein
wiskott- Aldrich syndrome protein-interacting protein
Wiskott-Aldrich syndrome protein-interacting protein
Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion.
Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
Involvement in disease
Note=A chromosomal aberration involving NCKIPSD/AF3p21 is found in therapy-related leukemia. Translocation t(3;11)(p21;q23) with MLL.
Contains 1 SH3 domain.
Nucleus. Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.