Recombinant Human NDEL1 protein (ab183232)
Key features and details
- Expression system: Escherichia coli
- Purity: > 80% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
Description
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Product name
Recombinant Human NDEL1 protein -
Purity
> 80 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMDGEDIP DFSSLKEETA YWKELSLKYK QSFQEARDEL VEFQEGSREL EAELEAQLVQ AEQRNRDLQA DNQRLKYEVE ALKEKLEHQY AQSYKQVSVL EDDLSQTRAI KEQLHKYVRE LEQANDDLER AKRATIVSLE DFEQRLNQAI ERNAFLESEL DEKESLLVSV QRLKDEARDL RQELAVRERQ QEVTRKSAPS SPTLDCEKMD SAVQASLSLP ATPVGKGTEN TFPSPKAIPN GFGTSPLTPS ARISALNIVG DLLRKVGALE SKLAACRNFA KDQASRKSYI SGNVNCGVLN GNGTKFSRSG HTSFFDKGQE KVIFPTLFMG Q -
Predicted molecular weight
39 kDa including tags -
Amino acids
1 to 328 -
Tags
His tag N-Terminus -
Additional sequence information
NP_001020750
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab183232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Additional notes
This product was previously labelled as Nudel
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 0.88% Sodium chloride, 10% Glycerol (glycerin, glycerine), 0.02% DTT
General Info
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Alternative names
- A. nidulans
- DKFZp451M0318
- ENDOOLIGOPEPTIDASE A
see all -
Function
Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. -
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
Sequence similarities
Belongs to the nudE family. -
Developmental stage
Expression peaks in mitosis. -
Post-translational
modificationsPhosphorylated in mitosis. Can be phosphorylated by CDK1, CDK5 and MAPK1. Phosphorylation by CDK5 promotes interaction with KATNA1 and YWHAE. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity). Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183232 has not yet been referenced specifically in any publications.