The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
NEDD4 family interacting protein 2
NEDD4 family-interacting protein 2
NEDD4 WW domain binding protein 5A
NEDD4 WW domain-binding protein 5A
Putative MAPK activating protein PM04/PM05/PM06/PM07
Putative MAPK-activating protein PM04/PM05/PM06/PM07
Putative NF kappa B activating protein 413
Putative NF-kappa-B-activating protein 413
Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling.
Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta.
The PY (WW-binding) motifs are required for E3 ubiquitin-protein ligase activation and for ubiquitination.
Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover. Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Also phosphorylated by LYN and FYN.
Endosome membrane. Golgi apparatus membrane. Endosome > multivesicular body membrane.