The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein concentration is above or equal to 0.05 mg/ml.
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Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00 Constituents: 0.31% Glutathione, 0.79% Tris HCl
Bifunctional heparan sulfate N deacetylase/N sulfotransferase 3
Glucosaminyl N deacetylase/N sulfotransferase 3
Glucosaminyl N-deacetylase/N-sulfotransferase 3
Heparan sulfate N-sulfotransferase 3
N deacetylase/N sulfotransferase (heparan glucosaminyl) 3
N heparan sulfate sulfotransferase 3
N HSST 3
N-heparan sulfate sulfotransferase 3
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has high deacetylase activity but low sulfotransferase activity.
Expressed in brain, kidney, liver, fetal and adult lung, adult pancreas, placenta, fetal spleen and fetal thymus. Not detected in adult/ fetal heart and skeletal muscle.