Recombinant human Nectin 3 protein (Fc Chimera Active) (ab221336)

Overview

Description

  • Nature

    Recombinant
  • Source

    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      PI IVEPHVTAVW GKNVSLKCLI EVNETITQIS WEKIHGKSSQ TVAVHHPQYG FSVQGEYQGR VLFKNYSLND ATITLHNIGF SDSGKYICKA VTFPLGNAQS STTVTVLVEP TVSLIKGPDS LIDGGNETVA AICIAATGKP VAHIDWEGDL GEMESTTTSF PNETATIISQ YKLFPTRFAR GRRITCVVKH PALEKDIRYS FILDIQYAPE VSVTGYDGNW FVGRKGVNLK CNADANPPPF KSVWSRLDGQ WPDGLLASDN TLHFVHPLTF NYSGVYICKV TNSLGQRSDQ KVIYISDPPT TTTLQPTIQW HPSTADIEDL ATEPKKLPFP LSTLATIKDD P KSCDKTHTCP PCPAPELLGG PSVFLFPPKP KDTLMISRTP EVTCVVVDVS HEDPEVKFNW YVDGVEVHNA KTKPREEQYN STYRVVSVLT VLHQDWLNGK EYKCKVSNKA LPAPIEKTIS KAKGQPREPQ VYTLPPSRDE LTKNQVSLTC LVKGFYPSDI AVEWESNGQP ENNYKTTPPV LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK
    • Molecular weight

      64 kDa including tags
    • Amino acids

      58 to 400
    • Additional sequence information

      ab221336 has a Human IgG1 Fc tag at the C-terminus (Uniprot# P01857, aa100-300)

Specifications

Our Abpromise guarantee covers the use of ab221336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Biological activity

    Immobilized Human Nectin 3, Fc Tag at 10 μg/mL (100 μL/well) can bind Biotinylated Human TIGIT, His Tag with a linear range of 0.3-5 μg/mL

  • Applications

    SDS-PAGE

    Functional Studies

  • Endotoxin level

    < 1.000 Eu/ml
  • Purity

    > 95 % SDS-PAGE.

  • Form

    Lyophilised
  • Additional notes

    No activity loss was observed after storage at:
    In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C).

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.5
    Constituents: 0.61% Tris, 0.75% Glycine, 5% Trehalose

    Lyophilized from 0.22 µm filtered solution

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    It is recommended to reconstitute the lyophilized product with sterile deionized water to a final concentration of 1 mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names

    • CD113
    • CDw113
    • DKFZP566B0846
    • FLJ90624
    • Nectin-3
    • Nectin3
    • Poliovirus receptor related 3
    • Poliovirus receptor related protein 3
    • Poliovirus receptor-like 3
    • Poliovirus receptor-related protein 3
    • PPR3
    • PRR3
    • Pvrl3
    • PVRL3_HUMAN
    • PVRR3
    see all
  • Function

    Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body.
  • Tissue specificity

    Predominantly expressed in testis and placenta as well as in many cell lines, including epithelial cell lines.
  • Sequence similarities

    Belongs to the nectin family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane.
  • Information by UniProt

Images

  • Human Nectin-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
  • Immobilized Human Nectin-1, His Tag at 2 µg/mL (100 µL/well) can bind Human Nectin-3, Fc Tag with a linear range of 5-78 ng/mL.
  • SDS-PAGE analysis of ab221336.

    The gel was stained overnight with Coomassie Blue. ab221336 has a calculated MW of 64 kDa. Due to glycosylation. The protein migrates at80-100 kDa under reducing conditions.

  • Immobilized ab221336 at 10 μg/mL (100 μL/well) biotinylated Human TIGIT, His Tag  with a linear range of 0.3-5 μg/mL.

References

ab221336 has not yet been referenced specifically in any publications.

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