The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
>95% by SDS-PAGE .
ab190449 is modified with fluorescein via primary amine coupling. This results in multiple fluoresceinated NEDD8 species with modified lysines as well as the N-terminus.
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Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Neural precursor cell expressed developmentally down regulated 8
Neural precursor cell expressed developmentally down regulated gene 8
Neural precursor cell expressed developmentally down-regulated protein 8
Ubiquitin like protein Nedd 8
Ubiquitin like protein Nedd8
Ubiquitin-like protein Nedd8
Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Highly expressed in heart, skeletal muscle, spleen, thymus, prostate, testis, ovary, colon and leukocytes.
Belongs to the ubiquitin family.
Cleavage of precursor form by UCHL3 or SENP8 is necessary for function.