Recombinant Human NEIL1 protein (ab123204)

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Overview

  • Product name

    Recombinant Human NEIL1 protein

  • Protein length

    Full length protein

Description

  • Nature

    Recombinant

  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
      Q96FI4

    • Species

      Human

    • Sequence

      MGSSHHHHHH SSGLVPRGSH MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS

    • Molecular weight

      46 kDa including tags

    • Amino acids

      1 to 390

    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab123204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Mass Spectrometry

    SDS-PAGE

  • Mass spectrometry

    MALDI-TOF

  • Purity

    > 90 % SDS-PAGE.
    ab123204 is purified using conventional chromatography.

  • Form

    Liquid

  • Additional notes

    EC=3.2.2.
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.002% PMSF, 0.02% DTT, 0.32% Tris HCl, 20% Glycerol, 0.58% Sodium chloride

General Info

  • Alternative names

    • DNA (apurinic or apyrimidinic site) lyase Neil1
    • DNA glycosylase/AP lyase Neil 1
    • DNA glycosylase/AP lyase Neil1
    • DNA-(apurinic or apyrimidinic site) lyase Neil1
    • Endonuclease 8 like 1
    • Endonuclease 8-like 1
    • Endonuclease VIII
    • Endonuclease VIII like 1
    • Endonuclease VIII-like 1
    • FLJ22402
    • FPG1
    • hFPG1
    • NEH 1
    • NEH1
    • NEI 1
    • Nei endonuclease VIII like 1
    • Nei endonuclease VIII like 1 (E. coli)
    • Nei homolog 1
    • Nei like 1
    • Nei like protein 1
    • Nei-like protein 1
    • NEI1
    • NEIL 1
    • Neil1
    • NEIL1_HUMAN
    see all

  • Function

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the FPG family.

  • Cellular localization

    Nucleus.
  • Information by UniProt

Images

  • SDS-PAGE - Recombinant Human NEIL1 protein (ab123204)
    SDS-PAGE - Recombinant Human NEIL1 protein (ab123204)
    15% SDS-PAGE analysis of ab123204 (3ug)

References

ab123204 has not yet been referenced specifically in any publications.

Publishing research using ab123204? Please let us know so that we can cite the reference in this datasheet.

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