Recombinant Human NEIL1 protein (ab123204)

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SDS-PAGE - Recombinant Human NEIL1 protein (ab123204)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 90% SDS-PAGE
    • Tags: His tag N-Terminus
    • Suitable for: MS, SDS-PAGE

    Description

    • Product name

      Recombinant Human NEIL1 protein

    • Purity

      > 90 % SDS-PAGE.
      ab123204 is purified using conventional chromatography.

    • Expression system

      Escherichia coli

    • Accession

      Q96FI4

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MGSSHHHHHH SSGLVPRGSH MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS

      • Predicted molecular weight

        46 kDa including tags

      • Amino acids

        1 to 390

      • Tags

        His tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab123204 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Mass Spectrometry

      SDS-PAGE

    • Mass spectrometry

      MALDI-TOF

    • Form

      Liquid

    • Additional notes

      EC=3.2.2.
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.002% PMSF, 0.02% DTT, 0.32% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride

    General Info

    • Alternative names

      • DNA (apurinic or apyrimidinic site) lyase Neil1
      • DNA glycosylase/AP lyase Neil 1
      • DNA glycosylase/AP lyase Neil1
      • DNA-(apurinic or apyrimidinic site) lyase Neil1
      • Endonuclease 8 like 1
      • Endonuclease 8-like 1
      • Endonuclease VIII
      • Endonuclease VIII like 1
      • Endonuclease VIII-like 1
      • FLJ22402
      • FPG1
      • hFPG1
      • NEH 1
      • NEH1
      • NEI 1
      • Nei endonuclease VIII like 1
      • Nei endonuclease VIII like 1 (E. coli)
      • Nei homolog 1
      • Nei like 1
      • Nei like protein 1
      • Nei-like protein 1
      • NEI1
      • NEIL 1
      • Neil1
      • NEIL1_HUMAN
      see all

    • Function

      Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.

    • Tissue specificity

      Ubiquitous.

    • Sequence similarities

      Belongs to the FPG family.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human NEIL1 protein (ab123204)
      SDS-PAGE - Recombinant Human NEIL1 protein (ab123204)
      15% SDS-PAGE analysis of ab123204 (3ug)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab123204? Please let us know so that we can cite the reference in this datasheet.

    ab123204 has not yet been referenced specifically in any publications.

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