Recombinant human NEK1 protein (ab133146)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human NEK1 protein
See all NEK1 proteins and peptides -
Biological activity
The specific activity of ab133146 was determined to be 70 nmol/min/mg. -
Purity
> 70 % Densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Predicted molecular weight
57 kDa -
Amino acids
1 to 495
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab133146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
Functional Studies
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Form
Liquid -
Additional notes
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DKFZp686D06121
- DKFZp686K12169
- kat
see all -
Function
Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis (By similarity). Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death. -
Tissue specificity
High fetal expression in the brain and kidney. -
Involvement in disease
Short-rib thoracic dysplasia 6 with or without polydactyly -
Sequence similarities
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associated with the pericentriolar material. Localizes to centrosome during interphase and mitosis (By similarity). - Information by UniProt
Images
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Functional Studies - Recombinant human NEK1 protein (ab133146)The specific activity of NEK1 (ab133146) was determined to be 80 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant human NEK1 protein (ab133146)SDS PAGE analysis of ab133146 -
SDS-PAGE - Recombinant human NEK1 protein (ab133146)SDS PAGE analysis of ab133146 -
Functional Studies - Recombinant human NEK1 protein (ab133146)The specific activity of ab133146 was determined to be 70 nmol/min/mg. -
SDS-PAGE - Recombinant human NEK1 protein (ab133146)SDS-PAGE analysis of ab133146.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133146 has not yet been referenced specifically in any publications.