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    recombinant-human-nek1-protein-ab133146.pdf

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Recombinant human NEK1 protein (ab133146)

  • Datasheet
  • SDS
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Functional Studies - Recombinant human NEK1 protein (ab133146)
  • SDS-PAGE - Recombinant human NEK1 protein (ab133146)
  • SDS-PAGE - Recombinant human NEK1 protein (ab133146)
  • Functional Studies - Recombinant human NEK1 protein (ab133146)
  • SDS-PAGE - Recombinant human NEK1 protein (ab133146)

Key features and details

  • Expression system: Baculovirus infected Sf9 cells
  • Purity: > 70% Densitometry
  • Active: Yes
  • Suitable for: WB, SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human NEK1 protein
    See all NEK1 proteins and peptides
  • Biological activity

    The specific activity of ab133146 was determined to be 70 nmol/min/mg.
  • Purity

    > 70 % Densitometry.

  • Expression system

    Baculovirus infected Sf9 cells
  • Accession

    Q96PY6
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Predicted molecular weight

      57 kDa
    • Amino acids

      1 to 495

Associated products

  • Substrate reagent

    • Native Pig Myelin Basic Protein (ab64311)

Specifications

Our Abpromise guarantee covers the use of ab133146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Additional notes

    ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • DKFZp686D06121
    • DKFZp686K12169
    • kat
    • KIAA1901
    • MGC138800
    • MGC189817
    • NEK1
    • NEK1_HUMAN
    • Never in mitosis A-related kinase 1
    • Never in mitosis gene A-related kinase 1
    • NIMA (never in mitosis gene a)-related kinase 1
    • NIMA-related kinase 1
    • NimA-related protein kinase 1
    • NY-REN-55
    • OTTHUMP00000219432
    • OTTHUMP00000219433
    • OTTHUMP00000219434
    • OTTHUMP00000219435
    • Protein-serine/threonine kinase
    • Renal carcinoma antigen NY-REN-55
    • Serine/threonine-protein kinase Nek1
    • SRPS2
    • SRPS2A
    • SRTD6
    see all
  • Function

    Phosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis (By similarity). Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death.
  • Tissue specificity

    High fetal expression in the brain and kidney.
  • Involvement in disease

    Short-rib thoracic dysplasia 6 with or without polydactyly
  • Sequence similarities

    Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associated with the pericentriolar material. Localizes to centrosome during interphase and mitosis (By similarity).
  • Target information above from: UniProt accession Q96PY6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

Images

  • Functional Studies - Recombinant human NEK1 protein (ab133146)
    Functional Studies - Recombinant human NEK1 protein (ab133146)
    The specific activity of NEK1 (ab133146) was determined to be 80 nmol/min/mg as per activity assay protocol
  • SDS-PAGE - Recombinant human NEK1 protein (ab133146)
    SDS-PAGE - Recombinant human NEK1 protein (ab133146)
    SDS PAGE analysis of ab133146
  • SDS-PAGE - Recombinant human NEK1 protein (ab133146)
    SDS-PAGE - Recombinant human NEK1 protein (ab133146)
    SDS PAGE analysis of ab133146
  • Functional Studies - Recombinant human NEK1 protein (ab133146)
    Functional Studies - Recombinant human NEK1 protein (ab133146)
    The specific activity of ab133146 was determined to be 70 nmol/min/mg.
  • SDS-PAGE - Recombinant human NEK1 protein (ab133146)
    SDS-PAGE - Recombinant human NEK1 protein (ab133146)
    SDS-PAGE analysis of ab133146.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab133146? Please let us know so that we can cite the reference in this datasheet.

ab133146 has not yet been referenced specifically in any publications.

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