Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Densitometry
- Active: Yes
- Suitable for: WB, SDS-PAGE, Functional Studies
Product nameRecombinant human NEK1 protein
See all NEK1 proteins and peptides
Biological activityThe specific activity of ab133146 was determined to be 70 nmol/min/mg.
Purity> 70 % Densitometry.
Expression systemBaculovirus infected Sf9 cells
Protein lengthProtein fragment
Predicted molecular weight57 kDa
Amino acids1 to 495
Our Abpromise guarantee covers the use of ab133146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab64311 (Myelin Basic Protein protein) can be utilized as a substrate for assessing kinase activity
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.005% DTT, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
FunctionPhosphorylates serines and threonines, but also appears to possess tyrosine kinase activity. Implicated in the control of meiosis (By similarity). Involved in cilium assembly. In response to injury that includes DNA damage, NEK1 phosphorylates VDAC1 to limit mitochondrial cell death.
Tissue specificityHigh fetal expression in the brain and kidney.
Involvement in diseaseShort-rib thoracic dysplasia 6 with or without polydactyly
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
Cellular localizationNucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Associated with the pericentriolar material. Localizes to centrosome during interphase and mitosis (By similarity).
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab133146 has not yet been referenced specifically in any publications.