Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 72% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human NEK7 protein
See all NEK7 proteins and peptides
Specific Activity: 47 pmol/min/µg
Assay was performed in a 25 µl reaction containing 40 mM Tris, pH 7.4, 20 mM MgCl2, 0.1 mg/ml BSA, 100 µM ATP, 0.2 mg/ml Casein and ab196067 for 50 min at 30°C.
Purity>= 72 % SDS-PAGE.
Expression systemBaculovirus infected Sf9 cells
Protein lengthFull length protein
SequenceMDEQSQGMQGPPVPQFQPQKALRPDMGYNTLANFRIEKKIGRGQFSEVYR AACLLDGVPVALKKVQIFDLMDAKARADCIKEIDLLKQLNHPNVIKYYAS FIEDNELNIVLELADAGDLSRMIKHFKKQKRLIPERTVWKYFVQLCSALE HMHSRRVMHRDIKPANVFITATGVVKLGDLGLGRFFSSKTTAAHSLVGTP YYMSPERIHENGYNFKSDIWSLGCLLYEMAALQSPFYGDKMNLYSLCKKI EQCDYPPLPSDHYSEELRQLVNMCINPDPEKRPDVTYVYDVAKRMHACTA SS
Predicted molecular weight61 kDa including tags
Amino acids1 to 302
TagsGST tag N-Terminus
Additional sequence informationNM_133494.
Our Abpromise guarantee covers the use of ab196067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.49% Glutathione, 0.05% DTT, 20% Glycerol
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- EC 22.214.171.124
Tissue specificityHighly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine.
Sequence similaritiesBelongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab196067 has not yet been referenced specifically in any publications.