Overview

  • Product name

    Recombinant Human NENF protein
  • Protein length

    Full length protein

Description

  • Nature

    Recombinant
  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species

      Human
    • Sequence

      MKHHHHHHAS GQTPRPAERG PPVRLFTEEE LARYGGEEED QPIYLAVKGV VFDVTSGKEF YGRGAPYNAL TGKDSTRGVA KMSLDPADLT HDTTGLTAKE LEALDEVFTK VYKAKYPIVG YTARRILNED GSPNLDFKPE DQPHFDIKDE F
    • Molecular weight

      16 kDa
    • Amino acids

      31 to 172
    • Tags

      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab139226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

    ELISA

  • Purity

    > 95 % SDS-PAGE.
    ab139226 is purified using Ni-NTA chromatography.
  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.40
    Constituents: 99% Phosphate Buffer, 0.43% Sodium chloride

  • Reconstitution
    Add 200µl of deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

General Info

  • Alternative names

    • Cell growth inhibiting protein 47
    • Cell immortalization-related protein 2
    • CIR2
    • Nenf
    • NENF_HUMAN
    • Neudesin
    • Neuron derived neurotrophic factor
    • Neuron-derived neurotrophic factor
    • SCIRP10
    • SCIRP10 related protein
    • Secreted protein of unknown function
    • Spinal cord injury related protein 10
    • SPUF
    • SPUF protein
    see all
  • Function

    Displays neurotrophic activity and activates phosphorylation of MAPK1/ERK2, MAPK3/ERK1 and AKT1/AKT in primary cultured neurons. Does not have mitogenic activity in primary cultured astrocytes. May play a role on neuronal differentiation and may have a transient effect on neural cell proliferation in neural precursor cells. Neurotrophic activity is enhanced by binding to heme.
  • Sequence similarities

    Belongs to the cytochrome b5 family. MAPR subfamily.
    Contains 1 cytochrome b5 heme-binding domain.
  • Domain

    The cytochrome b5 heme-binding domain was proven to bind heme, although it lacks the conserved iron-binding His residue at position 82.
  • Cellular localization

    Secreted > extracellular space.
  • Information by UniProt

Images

  • 14% SDS-PAGE separation of Human NENF
    Left: M.W. marker – 97, 66, 45, 31, 21, 14 kDa
    Lane 1: reduced and boiled sample, 2.55μg/lane.
    Lane 2: non-reduced and non-boiled sample, 2.55μg/lane.

References

ab139226 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.

The NENF protein (His tag), ab139226, has as you have observed, been expressed and purified from E. coli. As we have not performed any in vitro assays with this protein as yet, we cannot say if the protein will be active or not. However, if you would like to try the protein in your assays I would be able to offer you a testing discount.

This offer means that if you purchase ab139226 as normal, test the protein with your mouse in vitro assay and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for the equivalent price of ab139226 off any product in our catalogue. More information on this offer can be found here:

www.abcam.com/collaborationdiscount

Please note that the product to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab139226.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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