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I was thinking of purchasing your Nenf protein to use in some in vivo and in vitro assays. I have noticed that you have synthethized it in E. coli. Do you know if this protein suffers post-transcriptional modifications that limits its application for example in a murine model?
Have somebody already purchased it for investigation purposes? Do you know if it worked?
Asked on Oct 04 2012
Thank you for contacting us and your interest in our products.
The NENF protein (His tag), ab139226, has as you have observed, been expressed and purified from E. coli. As we have not performed any in vitro assays with this protein as yet, we cannot say if the protein will be active or not. However, if you would like to try the protein in your assays I would be able to offer you a testing discount.
This offer means that if you purchase ab139226 as normal, test the protein with your mouse in vitro assay and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for the equivalent price of ab139226 off any product in our catalogue. More information on this offer can be found here:
Please note that the product to be tested must be purchased, tested, the Abreview submitted and the free product claimed within a 4 month period.
If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab139226.
I hope this information has been of help. If you require any further information please do not hesitate to contact us again.
Answered on Oct 04 2012