Recombinant Human Neurofilament heavy polypeptide protein (ab158986)
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- References
- Protocols
Overview
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Product nameRecombinant Human Neurofilament heavy polypeptide protein
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Protein lengthProtein fragment
Description
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NatureRecombinant
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SourceWheat germ
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Amino Acid Sequence
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SpeciesHuman
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SequenceLKCDVTSALREIRAQLEGHAVQSTLQSEEWFRVRLDRLSEAAKVNTDAMR SAQEEITEYRRQLQARTTELEALKSTKDSLERQRSELEDRHQADIASYQE A
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Amino acids263 to 363
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TagsGST tag N-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab158986 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
ELISA
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FormLiquid
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Additional notes
Protein concentration is above or equal to 0.05 mg/ml.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
General Info
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Alternative names
- 200 kDa neurofilament protein
- CMT2CC
- Nefh
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FunctionNeurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins.
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Involvement in diseaseDefects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors.
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Sequence similaritiesBelongs to the intermediate filament family.
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Post-translational
modificationsThere are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human Neurofilament heavy polypeptide protein (ab158986)ab158986 on a 12.5% SDS-PAGE stained with Coomassie Blue.
Datasheets and documents
References
ab158986 has not yet been referenced specifically in any publications.
