Key features and details
- Expression system: Insect cells
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Product nameRecombinant human Neuropilin 1 protein
See all Neuropilin 1 proteins and peptides
Measured by its binding ability in a functional ELISA. Immobilized soluble Neuropilin 1 binds all VEGF-A isoforms with the exception of VEGF.
Purity> 95 % SDS-PAGE.
Expression systemInsect cells
Protein lengthProtein fragment
SequenceFRNDKCGDTIKIESPGYLTSPGYPHSYHPSEKCEWLIQAPDPYQRIMINF NP HFDLEDRDCKYDYVEVFDGENENGHFRGKFCGKIAPPPVVSSGPFL FIKFVS DYETHGAGFSIRYEIFKRGPECSQNYTTPSGVIKSPGFPEKY PNSLECTYIV FAPKMSEIILEFESFDLEPDSNPPGGMFCRYDRLEIWD GFPDVGPHIGRYCG QKTPGRIRSSSGILSMVFYTDSAIAKEGFSANYS VLQSSVSEDFKCMEALGM ESGEIHSDQITASSQYSTNWSAERSRLNYP ENGWTPGEDSYREWIQVDLGLL RFVTAVGTQGAISKETKKKYYVKTYK IDVSSNGEDWITIKEGNKPVLFQGNT NPTDVVVAVFPKPLITRFVRIK PATWETGISMRFEVYGCKITDYPCSGMLGM VSGLISDSQITSSNQGDR NWMPENIRLVTSRSGWALPPAPHSYINEWLQIDL GEEKIVRGIIIQGG KHRENKVFMRKFKIGYSNNGSDWKMIMDDSKRKAKSFE GNNNYDTPEL RTFPALSTRFIRIYPERATHGGLGLRMELLGCEVEAPTAGPT TPNGNL VDECDDDQANCHSGTGDDFQLTGGTTVLATEKPTVIDSTIQSGIKL EH HHHHH
Predicted molecular weight83 kDa including tags
Amino acids22 to 641
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab204616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituent: 100% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionReconstitute in water or PBS at a concentration of not lower than 100 µg/ml.
- A5 protein
- BLOOD DENDRITIC CELL ANTIGEN 4
FunctionThe membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis.
The soluble isoform 2 binds VEGF-165 and appears to inhibit its binding to cells. It may also induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Tissue specificityThe expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules.
Sequence similaritiesBelongs to the neuropilin family.
Contains 2 CUB domains.
Contains 2 F5/8 type C domains.
Contains 1 MAM domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab204616 has not yet been referenced specifically in any publications.