Recombinant Human Neuroserpin protein (ab167956)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      MKHHHHHHAS TGATFPEEAI ADLSVNMYNR LRATGEDENI LFSPLSIALA MGMMELGAQG STQKEIRHSM GYDSLKNGEE FSFLKEFSNM VTAKESQYVM KIANSLFVQN GFHVNEEFLQ MMKKYFNAAV NHVDFSQNVA VANYINKWVE NNTNNLVKDL VSPRDFDAAT YLALINAVYF KGNWKSQFRP ENTRTFSFTK DDESEVQIPM MYQQGEFYYG EFSDGSNEAG GIYQVLEIPY EGDEISMMLV LSRQEVPLAT LEPLVKAQLV EEWANSVKKQ KVEVYLPRFT VEQEIDLKDV LKALGITEIF IKDANLTGLS DNKEIFLSKA IHKSFLEVNE EGSEAAAVSG MIAISRMAVL YPQVIVDHPF FFLIRNRRTG TILFMGRVMH PETMNTSGHD FEEL
    • Molecular weight
      46 kDa including tags
    • Amino acids
      17 to 410
    • Tags
      His tag N-Terminus

Specifications

Our Abpromise guarantee covers the use of ab167956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    ELISA

    SDS-PAGE

    Mass Spectrometry

  • Endotoxin level
    < 1.000 Eu/µg
  • Mass spectrometry
    LC-MS/MS
  • Purity
    > 95 % Densitometry.
    ab167956 was purifed using Ni-NTA chromatography and filtered (0.4 µm).
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C.

    pH: 7.40
    Constituents: 99% Phosphate Buffer, 0.2% Sodium chloride

  • Reconstitution
    Add 200 µl of deionized water to prepare a working stock solution of 0.5 mg/ml and let the lyophilized pellet dissolve completely. Avoid repeated freezing/thawing cycles.

    Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

General Info

  • Alternative names
    • DKFZp781N13156
    • Neuroserpin
    • NEUS_HUMAN
    • Peptidase inhibitor 12
    • PI-12
    • PI12
    • Protease inhibitor 12
    • Serine or cysteine proteinase inhibitor clade I (neuroserpin) member 1
    • Serine or cysteine proteinase inhibitor clade I member 1
    • Serpin I1
    • Serpin peptidase inhibitor clade I (neuroserpin) member 1
    • SERPINI1
    see all
  • Function
    Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
  • Tissue specificity
    Predominantly expressed in the brain.
  • Involvement in disease
    Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin.
  • Sequence similarities
    Belongs to the serpin family.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • 12% SDS-PAGE analysis of ab167956:
    Lane 1: MWt marker
    Lane 2: non reduced and non boiled sample, 2.5 μg
    Lane 3: reduced and boiled sample, 2.5 μg

References

ab167956 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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