Recombinant human Neuroserpin protein (ab201432)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, HPLC, SDS-PAGE
Description
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Product name
Recombinant human Neuroserpin protein
See all Neuroserpin proteins and peptides -
Biological activity
Fully biologically active when compared to standard.
The ED50 determined by a cell proliferation assay using rat C6 cells is less than 600 ng/ml, corresponding to a specific activity of >1.67×103 IU/mg.
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Purity
> 95 % SDS-PAGE.
> 95 % by HPLC. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
TGATFPEEAIADLSVNMYNRLRATGEDENILFSPLSIALAMGMMELGAQG STQKEIRHSMGYDSLKNGEEFSFLKEFSNMVTAKESQYVMKIANSLFVQN GFHVNEEFLQMMKKYFNAAVNHVDFSQNVAVANYINKWVENNTNNLVKDL VSPRDFDAATYLALINAVYFKGNWKSQFRPENTRTFSFTKDDESEVQIPM MYQQGEFYYGEFSDGSNEAGGIYQVLEIPYEGDEISMMLVLSRQEVPLAT LEPLVKAQLVEEWANSVKKQKVEVYLPRFTVEQEIDLKDVLKALGITEIF IKDANLTGLSDNKEIFLSKAIHKSFLEVNEEGSEAAAVSGMIAISRMAVL YPQVIVDHPFFFLIRNRRTGTILFMGRVMHPETMNTSGHDFEEL -
Predicted molecular weight
45 kDa -
Amino acids
17 to 410 -
Additional sequence information
This product is for the mature full length protein. The signal peptide is not included.
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Specifications
Our Abpromise guarantee covers the use of ab201432 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
HPLC
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C long term. Avoid freeze / thaw cycle.
pH: 7.40
Constituent: 100% PBS
0.2µm filteredThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionBriefly centrifuge prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.
General Info
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Alternative names
- DKFZp781N13156
- Neuroserpin
- NEUS_HUMAN
see all -
Function
Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. -
Tissue specificity
Predominantly expressed in the brain. -
Involvement in disease
Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. -
Sequence similarities
Belongs to the serpin family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab201432 has not yet been referenced specifically in any publications.