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    recombinant-human-neuroserpin-protein-ab63224.pdf

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Recombinant Human Neuroserpin protein (ab63224)

  • Datasheet
  • SDS
Reviews (1)Q&A (3)References (1)

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Western blot - Recombinant Human Neuroserpin protein (ab63224)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 95% SDS-PAGE
    • Endotoxin level: < 0.100 Eu/µg
    • Suitable for: Functional Studies, SDS-PAGE, HPLC, WB

    Description

    • Product name

      Recombinant Human Neuroserpin protein
      See all Neuroserpin proteins and peptides
    • Purity

      > 95 % SDS-PAGE.
      Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
    • Endotoxin level

      < 0.100 Eu/µg
    • Expression system

      Escherichia coli
    • Protein length

      Full length protein
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL

    Associated products

    • Related Products

      • Anti-Neuroserpin antibody (ab33077)

    Specifications

    Our Abpromise guarantee covers the use of ab63224 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      SDS-PAGE

      HPLC

      Western blot

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    • Reconstitution
      For lot specific reconstitution information please contact our Scientific Support Team.

    General Info

    • Alternative names

      • DKFZp781N13156
      • Neuroserpin
      • NEUS_HUMAN
      • Peptidase inhibitor 12
      • PI-12
      • PI12
      • Protease inhibitor 12
      • Serine or cysteine proteinase inhibitor clade I (neuroserpin) member 1
      • Serine or cysteine proteinase inhibitor clade I member 1
      • Serpin I1
      • Serpin peptidase inhibitor clade I (neuroserpin) member 1
      • SERPINI1
      see all
    • Function

      Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
    • Tissue specificity

      Predominantly expressed in the brain.
    • Involvement in disease

      Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin.
    • Sequence similarities

      Belongs to the serpin family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession Q99574 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • Western blot - Recombinant Human Neuroserpin protein (ab63224)
      Western blot - Recombinant Human Neuroserpin protein (ab63224)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab63224? Please let us know so that we can cite the reference in this datasheet.

    ab63224 has been referenced in 1 publication.

    • Subhadra B  et al. Neuroserpin up-regulation in the Alzheimer's disease brain is associated with elevated thyroid hormone receptor-ß1 and HuD expression. Neurochem Int 63:476-81 (2013). PubMed: 24036060

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Question

    Per our conversation this morning I have included the graph of Ab #63224 E.coli recombinant Human Neuroserpin

    Lot # *** (1mg/ml)

    The product reacts with antibody to mouse neuroserpin.Unfortunately,your serpin fails to inhibit its primary protease target in the nervous system, ie.human tissue plasminogen activator, as seen in the attached graph.

    I would rate the product poorin terms of functional activity; albeit, good as an antigen target for neuroserpin antibodies.

    Thank youfor uploading this information and finalizing the AbReview.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 07 2012

    Answer

    Thank you for sending that information.

    I have gone ahead and uploaded all of that information into the Abreview and have also published the Abreview.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Mar 07 2012

    Other Abreview for Neuroserpin protein

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Other - Do not use

    Review text: Comparing the tPA inhibitory activity of Abcam Neuroserpin versus mouse neuroserpin

    Procedure: tPA proteolytic assay using

    Results:
    Mouse neuroserpin inhibited ~70% tPA activity in 5 minutes where as Abcam neuroserpin inhibited ~20 % of tPA activity in 5 minutes. A 1:100 molar ratio of
    Abcam NS to tPA gave no better inhibition, if anything it was less inhibitory.
    Sample: Escherichia coli

    Primary antibody (in addition to 'Neuroserpin protein')
    Primary antibody: None used

    Secondary antibody
    Secondary antibody: None used

    Abcam user community

    Verified customer

    Submitted Mar 07 2012

    Question

    We would be glad to test your E.coli recombinant Neuroserpin as an inhibitor of tissue plasminogen activator using a kinetic assay with either a chromogenic or flurogenic tPA substrate. We will let you know the results; hopefully for us it will inhibit tPA activity. Please send purchase code info prior to our purchase. Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on Jan 06 2012

    Answer

    DISCOUNT CODE: ************ Expiration date:************* I am very pleased to hear you would like to use ab63224 in an Inhibition Assayand send us an image of your results. This code will give you 1 free PROTEIN before the expiration date. To redeem this offer, please submit an Abreview with the image for Inhibition assay and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated. Remember if the product does not work you will be covered by our Abpromise guarantee and eligible for a full refund or replacement. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jan 06 2012

    Question

    Have you tested this protein to see if it directly inhibits TPA activity?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 06 2012

    Answer

    Thank you for contacting Abcam. We have not tested ab63224 to see if it inhibits TPA activity. We do not have sample sizes of our products, but what I can offer is out testing disount program. Asab63224 has not been tested in an inhibition assay, I can offer a discount off a future purchase if you buy ab63224 now, test it in inhibition assay and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PROTEIN. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab63224 in inhibition assay. I will then send a discount code. This code must be issued before purchasing ab63224 so please wait for my reply before ordering. 2. Purchase ab63224 either by phone, fax, or online (www.abcam.com). 3. Test it in inhibition assay. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PROTEIN ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab62334 turns out to be unsuitable for inhibition assay you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Jan 06 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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