Recombinant Human Neuroserpin protein (ab63224)

Abreviews (1)Q&A (3)Specific References (1)

Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Species
      Human
    • Sequence
      MTGATFPEEA IADLSVNMYN RLRATGEDEN ILFSPLSIAL AMGMMELGAQ GSTQKEIRHS MGYDSLKNGE EFSFLKEFSN MVTAKESQYV MKIANSLFVQ NGFHVNEEFL QMMKKYFNAA VNHVDFSQNV AVANYINKWV ENNTNNLVKD LVSPRDFDAA TYLALINAVY FKGNWKSQFR PENTRTFSFT KDDESEVQIP MMYQQGEFYY GEFSDGSNEA GGIYQVLEIP YEGDEISMML VLSRQEVPLA TLEPLVKAQL VEEWANSVKK QKVEVYLPRF TVEQEIDLKD VLKALGITEI FIKDANLTGL SDNKEIFLSK AIHKSFLEVN EEGSEAAAVS GMIAISRMAV LYPQVIVDHP FFFLIRNRRT GTILFMGRVM HPETMNTSGH DFEEL

Specifications

Our Abpromise guarantee covers the use of ab63224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Functional Studies

    SDS-PAGE

    HPLC

    Western blot

  • Endotoxin level
    < 0.100 Eu/µg
  • Purity
    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

General Info

  • Alternative names
    • DKFZp781N13156
    • Neuroserpin
    • NEUS_HUMAN
    • Peptidase inhibitor 12
    • PI-12
    • PI12
    • Protease inhibitor 12
    • Serine or cysteine proteinase inhibitor clade I (neuroserpin) member 1
    • Serine or cysteine proteinase inhibitor clade I member 1
    • Serpin I1
    • Serpin peptidase inhibitor clade I (neuroserpin) member 1
    • SERPINI1
    see all
  • Function
    Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator.
  • Tissue specificity
    Predominantly expressed in the brain.
  • Involvement in disease
    Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin.
  • Sequence similarities
    Belongs to the serpin family.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • Western blot - Recombinant Human Neuroserpin protein (ab63224)
    Western blot - Recombinant Human Neuroserpin protein (ab63224)

References

This product has been referenced in:
  • Subhadra B  et al. Neuroserpin up-regulation in the Alzheimer's disease brain is associated with elevated thyroid hormone receptor-ß1 and HuD expression. Neurochem Int 63:476-81 (2013). Read more (PubMed: 24036060) »

See 1 Publication for this product

Publishing research using ab63224? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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4 reviews or Q&A

10
Votes: Highest First

Per our conversation this morning I have included the graph of Ab #63224 E.coli recombinant Human Neuroserpin

Lot # *** (1mg/ml)

The product reacts with antibody to mouse neuroserpin.Unfortunately,your serpin fails to inhibit its ...

Read More

Thank you for sending that information.

I have gone ahead and uploaded all of that information into the Abreview and have also published the Abreview.

Please let me know if there is anything else I can help you with.

Other Abreview for Neuroserpin protein

 Poor
Abreviews
Application
Other

Review text: Comparing the tPA inhibitory activity of Abcam Neuroserpin versus mouse neuroserpin

Procedure: tPA proteolytic assay using

Results:
Mouse neuroserpin inhibited ~70% tPA activity in 5 minutes where as Abcam neuroserpin inhibited ~20 % of tPA activity in 5 minutes. A 1:100 molar ratio of
Abcam NS to tPA gave no better inhibition, if anything it was less inhibitory.
Sample: Escherichia coli

Primary antibody (in addition to 'Neuroserpin protein')
Primary antibody: None used

Secondary antibody
Secondary antibody: None used
abreview image
Username

Abcam user community

Verified customer

Submitted Mar 07 2012

We would be glad to test your E.coli recombinant Neuroserpin as an inhibitor of tissue plasminogen activator using a kinetic assay with either a chromogenic or flurogenic tPA substrate. We will let you know the results; hopefully for us it will inhibit...

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DISCOUNT CODE: ************ Expiration date:************* I am very pleased to hear you would like to use ab63224 in an Inhibition Assayand send us an image of your results. This code will give you 1 free PROTEIN before the expiration date. To redeem t...

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Have you tested this protein to see if it directly inhibits TPA activity?

Thank you for contacting Abcam. We have not tested ab63224 to see if it inhibits TPA activity. We do not have sample sizes of our products, but what I can offer is out testing disount program. Asab63224 has not been tested in an inhibition assay, I can...

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