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Cell Biology Apoptosis Intracellular NFkB RelA (p65)
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Recombinant Human NF-kB p65 protein (Tagged) (ab114150)

  • Datasheet
  • SDS
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SDS-PAGE - Recombinant Human NF-kB p65 protein (ab114150)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: WB, ELISA, SDS-PAGE

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    Description

    • Product name

      Recombinant Human NF-kB p65 protein (Tagged)
      See all NF-kB p65 proteins and peptides
    • Expression system

      Wheat germ
    • Accession

      Q04206
    • Protein length

      Protein fragment
    • Animal free

      No
    • Nature

      Recombinant
      • Species

        Human
      • Sequence

        MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGER STDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFY EAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRG DYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVN RNSGSCLGGDEIFLLCDKVQ
      • Predicted molecular weight

        51 kDa including tags
      • Amino acids

        1 to 220
      • Tags

        GST tag N-Terminus

    Associated products

    • Matched antibody pairs

      • Anti-NF-kB p65 (phospho S311) antibody (ab194926)
    • Related Products

      • Anti-GST antibody [EPR4236] (ab111947)
      • NF kappaB p65 ELISA Kit (ab176648)
      • Anti-GST antibody (ab19256)
      • Anti-GST antibody (ab9085)

    Specifications

    Our Abpromise guarantee covers the use of ab114150 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • Avian reticuloendotheliosis viral (v rel) oncogene homolog A
      • MGC131774
      • NF kappa B p65delta3
      • nfkappabp65
      • NFkB p65
      • NFKB3
      • Nuclear factor kappaB
      • Nuclear Factor NF Kappa B p65 Subunit
      • Nuclear factor NF-kappa-B p65 subunit
      • Nuclear factor of kappa light polypeptide gene enhancer in B cells 3
      • Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3
      • OTTHUMP00000233473
      • OTTHUMP00000233474
      • OTTHUMP00000233475
      • OTTHUMP00000233476
      • OTTHUMP00000233900
      • p65
      • p65 NF kappaB
      • p65 NFkB
      • relA
      • TF65_HUMAN
      • Transcription factor NFKB3
      • Transcription factor p65
      • V rel avian reticuloendotheliosis viral oncogene homolog A
      • v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65))
      • v rel reticuloendotheliosis viral oncogene homolog A (avian)
      • V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65
      see all
    • Function

      NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.
    • Sequence similarities

      Contains 1 RHD (Rel-like) domain.
    • Domain

      the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
    • Post-translational
      modifications

      Ubiquitinated, leading to its proteasomal degradation. Degradation is required for termination of NF-kappa-B response.
      Monomethylated at Lys-310 by SETD6. Monomethylation at Lys-310 is recognized by the ANK repeats of EHMT1 and promotes the formation of repressed chromatin at target genes, leading to down-regulation of NF-kappa-B transcription factor activity. Phosphorylation at Ser-311 disrupts the interaction with EHMT1 without preventing monomethylation at Lys-310 and relieves the repression of target genes.
      Phosphorylation at Ser-311 disrupts the interaction with EHMT1 and promotes transcription factor activity (By similarity). Phosphorylation on Ser-536 stimulates acetylation on Lys-310 and interaction with CBP; the phosphorylated and acetylated forms show enhanced transcriptional activity.
      Reversibly acetylated; the acetylation seems to be mediated by CBP, the deacetylation by HDAC3. Acetylation at Lys-122 enhances DNA binding and impairs association with NFKBIA. Acetylation at Lys-310 is required for full transcriptional activity in the absence of effects on DNA binding and NFKBIA association. Acetylation can also lower DNA-binding and results in nuclear export. Interaction with BRMS1 promotes deacetylation of 'Lys-310'.
    • Cellular localization

      Nucleus. Cytoplasm. Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). Colocalized with RELA in the nucleus upon TNF-alpha induction.
    • Target information above from: UniProt accession Q04206 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human NF-kB p65 protein (ab114150)
      SDS-PAGE - Recombinant Human NF-kB p65 protein (ab114150)
      12.5% SDS-PAGE analysis of ab114150 stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab114150? Please let us know so that we can cite the reference in this datasheet.

    ab114150 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Question

    How much protein was loaded into the SDS gel on the image on the datasheet?

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    Abcam community

    Verified customer

    Asked on Jul 23 2012

    Answer

    Thank you for contacting Abcam.

    For the SDS-Page gel image on the webpage, we loaded5ul of protein per lane.
    If there is anything else I can help you with, please let me know.

    Read More

    Abcam Scientific Support

    Answered on Jul 23 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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