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Recombinant Human NFAT4/NF-ATc3 protein (ab114667)

Submit a review Submit a question References (1)
SDS-PAGE - Recombinant Human NFAT4/NF-ATc3 protein (ab114667)

    Key features and details

    • Expression system: Wheat germ
    • Suitable for: WB, ELISA, SDS-PAGE

    Description

    • Product name

      Recombinant Human NFAT4/NF-ATc3 protein

    • Expression system

      Wheat germ

    • Accession

      Q12968

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        MTTANCGAHDELDFKLVFGEDGAPAPPPPGSRPADLEPDDCASIYIFNVD PPPSTLTTPLCLPHHGLPSHSSVLSPSFQLQSHKNYEGTCEIPESKYSPL GGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHLY LPLEPSYRESSLSPSPASSISSRSWFSDASSCESLSHIYDDVDSELNEAA ARFTLGSPLTSPGGSPGGCPGEETWHQQYGLGHSLSPRQSPCHSPRSSVT DENWLSPRPASGPSSRPTSPCGKRRHSSAEVCYAGSLSPHHSPVPSPGHS PRGSVTEDTWLNASVHGGSGLGPAVFPFQYCVETDIPLKTRKTSEDQAAI LPGKLELCSDDQGSLSPARETSIDDGLGSQYPLKKDSCGDQFLSVPSPFT WSKPKPGHTPIFRTSSLPPLDWPLPAHFGQCELKIEVQPKTHHRAHYETE GSRGAVKASTGGHPVVKLLGYNEKPINLQMFIGTADDRYLRPHAFYQVHR ITGKTVATASQEIIIASTKVLEIPLLPENNMSASIDCAGILKLRNSDIEL RKGETDIGRKNTRVRLVFRVHIPQPSGKVLSLQIASIPVECSQRSAQELP HIEKYSINSCSVNGGHEMVVTGSNFLPESKIIFLEKGQDGRPQWEVEGKI IREKCQGAHIVLEVPPYHNPAVTAAVQVHFYLCNGKRKKSQSQRFTYTPV LMKQEHREEIDLSSVPSLPVPHPAQTQRPSSDSGCSHDSVLSGQRSLICS IPQTYASMVTSSHLPQLQCRDESVSKEQHMIPSPIVHQPFQVTPTPPVGS SYQPMQTNVVYNGPTCLPINAASSQEFDSVLFQQDATLSGLVNLGCQPLS SIPFHSSNSGSTGHLLAHTPHSVHTLPHLQSMGYHCSNTGQRSLSSPVAD QITGQPSSQLQPITYGPSHSGSATTASPAASHPLASSPLSGPPSPQLQPM PYQSPSSGTASSPSPATRMHSGQHSTQAQSTGQGGLSAPSSLICHSLCDP ASFPPDGATVSIKPEPEDREPNFATIGLQDITLDDDQFISDLEHQPSGSA EKWPNHSVLSCPAPFWRI

      • Predicted molecular weight

        144 kDa

      • Amino acids

        1 to 1068

    Specifications

    Our Abpromise guarantee covers the use of ab114667 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Western blot

      ELISA

      SDS-PAGE

    • Form

      Liquid

    • Additional notes

      This product was previously labelled as NFAT4.

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.3% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • C80703
      • cytoplasmic 3
      • D8Ertd281e
      • NF AT4
      • NF ATc3
      • NF-AT4
      • NF-ATc3
      • NFAC3_HUMAN
      • NFAT4
      • NFATc3
      • NFATx
      • Nuclear factor of activated T cells, cytoplasmic 3
      • nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3
      • Nuclear factor of activated T-cells
      • T cell transcription factor NFAT4
      • T-cell transcription factor NFAT4
      see all

    • Function

      Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2.

    • Tissue specificity

      Isoform 1 is predominantly expressed in thymus and is also found in peripheral blood leukocytes and kidney. Isoform 2 is predominantly expressed in skeletal muscle and is also found in thymus, kidney, testis, spleen, prostate, ovary, small intestine, heart, placenta and pancreas. Isoform 3 is expressed in thymus and kidney. Isoform 4 is expressed in thymus and skeletal muscle.

    • Sequence similarities

      Contains 1 RHD (Rel-like) domain.

    • Domain

      Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors.

    • Post-translational
      modifications

      Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin.

    • Cellular localization

      Cytoplasm. Nucleus. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human NFAT4/NF-ATc3 protein (ab114667)
      SDS-PAGE - Recombinant Human NFAT4/NF-ATc3 protein (ab114667)
      SDS-PAGE analysis of ab114667 on a 12.5% gel stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab114667? Please let us know so that we can cite the reference in this datasheet.

    ab114667 has been referenced in 1 publication.

    • Samanta J  et al. Oleic Acid Protects from Arsenic-Induced Cardiac Hypertrophy via AMPK/FoxO/NFATc3 Pathway. Cardiovasc Toxicol 20:261-280 (2020). PubMed: 31571030

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