Key features and details
- Expression system: Escherichia coli
- Purity: >= 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, Functional Studies, SDS-PAGE
Product nameRecombinant human NGF protein (Active)
See all NGF proteins and peptides
Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.
The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Purity>= 98 % SDS-PAGE.
>=98% HPLC analyses. Sterile filtered.
Endotoxin level< 1.000 Eu/µg
Expression systemEscherichia coli
Protein lengthFull length protein
SequenceSSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRASSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
Predicted molecular weight27 kDa
Amino acids122 to 241
Additional sequence informationFull length mature protein, without the signal peptide or the propeptide. The functional form is a non-covalently linked homodimer 27 kDa.
Our Abpromise guarantee covers the use of ab9796 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionPlease reconstitute this product to 0.1-1 mg/ml in water. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA) for long term storage.
- Beta nerve growth factor
- Beta NGF
- Beta-nerve growth factor
FunctionNerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
Involvement in diseaseNeuropathy, hereditary sensory and autonomic, 5
Sequence similaritiesBelongs to the NGF-beta family.
- Information by UniProt
All lanes : Anti-TrkA (phospho Y496) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution
Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
Blocking and dilution buffer: 5% NFDM/TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab9796 has not yet been referenced specifically in any publications.