Description

  • Product name

    Recombinant human NGF protein (Active)
    See all NGF proteins and peptides
  • Biological activity

    Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells.

    The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.

  • Purity

    >= 98 % SDS-PAGE.
    >=98% HPLC analyses. Sterile filtered.
  • Endotoxin level

    < 1.000 Eu/µg
  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRASSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVRRA
    • Predicted molecular weight

      27 kDa
    • Amino acids

      122 to 241
    • Additional sequence information

      Full length mature protein, without the signal peptide or the propeptide. The functional form is a non-covalently linked homodimer 27 kDa.

Associated products

Specifications

Our Abpromise guarantee covers the use of ab9796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    HPLC

    Functional Studies

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    n/a

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

  • Reconstitution
    Please reconstitute this product to 0.1-1 mg/ml in water. Follow reconstitution with further dilution in a buffer containing a carrier protein (example 0.1% BSA) for long term storage.

General Info

  • Alternative names

    • Beta nerve growth factor
    • Beta NGF
    • Beta-nerve growth factor
    • Beta-NGF
    • HSAN5
    • MGC161426
    • MGC161428
    • Nerve growth factor
    • Nerve growth factor (beta polypeptide)
    • Nerve growth factor beta
    • Nerve growth factor beta polypeptide
    • Nerve growth factor beta subunit
    • NGF
    • NGF_HUMAN
    • NGFB
    • NID67
    see all
  • Function

    Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177).
  • Involvement in disease

    Neuropathy, hereditary sensory and autonomic, 5
  • Sequence similarities

    Belongs to the NGF-beta family.
  • Cellular localization

    Secreted.
  • Information by UniProt

Images

  • All lanes : Anti-TrkA (phospho Y490) + TrkB (phospho Y516) + TrkC (phospho Y516) antibody [EPR19140] (ab197071) at 1/1000 dilution

    Lane 1 : Untreated PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 2 : PC-12 treated with 100 ng/mL NGF (ab9796) for 5 minutes, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Observed band size: 140 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

References

ab9796 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Ab9796 is a noncovalently disulfide-linked homodimer, of two 13.5kDa polypeptide monomers (238 total amino acid residues). The three disulfide bonds are required for biological activity.

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Answer

As you have probably seen from the online datasheet, ab9796 -- NGF beta (human) recombinant protein -- is not currently in stock. The lead time for this item would be approx 7 working days. If you were to order 6 units on a single purchase order I could offer you a 10% discount on the regular price of USD 293 for 20µg. Unfortunately we do not have the facilities to deal with a weekly standing order. I would recommend that you order sufficient quantities of this product for your research and if you require more than 6 units for your whole study, I can investigate the possibility of a larger bulk discount.

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