Recombinant human NGF protein (Animal Free) (ab217472)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
Description
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Product name
Recombinant human NGF protein (Animal Free)
See all NGF proteins and peptides -
Biological activity
Determined by the dose-dependent stimulation of the proliferation of human TF-1 cells. The expected ED50 is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
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Purity
> 98 % SDS-PAGE.
> 98 % by HPLC. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
SSSHPIFHRG EFSVCDSVSV WVGDKTTATD IKGKEVMVLG EVNINNSVFK QYFFETKCRD PNPVDSGCRG IDSKHWNSYC TTTHTFVKAL TMDGKQAAWR FIRIDTACVC VLSRKAVR -
Predicted molecular weight
13 kDa -
Amino acids
122 to 239 -
Additional sequence information
A non-covalently-linked homodimer of two 13.5 kDa, polypeptide monomers that each contain 120 amino acids and three disulfide bonds.
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Specifications
Our Abpromise guarantee covers the use of ab217472 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
HPLC
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionFor lot specific reconstitution information please contact our Scientific Support Team.
General Info
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Alternative names
- Beta nerve growth factor
- Beta NGF
- Beta-nerve growth factor
see all -
Function
Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). -
Involvement in disease
Neuropathy, hereditary sensory and autonomic, 5 -
Sequence similarities
Belongs to the NGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab217472 has not yet been referenced specifically in any publications.