Recombinant Human NHP2 protein (ab102027)
- Datasheet
- References
- Protocols
Description
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Product name
Recombinant Human NHP2 protein
See all NHP2 proteins and peptides -
Purity
> 90 % SDS-PAGE.
ab102027 was purified using conventional chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMTKIKADPDGPEAQAEACSGERTYQELLVN QNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKFVNKGEKGIMV LAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVK PHEEYQEAYDECLEEVQSLPLPL -
Predicted molecular weight
19 kDa including tags -
Amino acids
1 to 153 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab102027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 20% Glycerol, 0.58% Sodium chloride
General Info
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Alternative names
- DKCB2
- FLJ20479
- H/ACA ribonucleoprotein complex subunit 2
see all -
Function
Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. -
Tissue specificity
Expressed in brain, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen, testis and thymus. Also expressed at lower levels in the liver. -
Involvement in disease
Dyskeratosis congenita, autosomal recessive, 2 -
Sequence similarities
Belongs to the ribosomal protein L7Ae family. -
Developmental stage
Transcript peaks at G1/S transition. -
Cellular localization
Nucleus > nucleolus. Nucleus > Cajal body. Also localized to Cajal bodies. - Information by UniProt
Images
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SDS-PAGE - Recombinant Human NHP2 protein (ab102027)15% SDS-PAGE analysis of ab102027 (3µg)
Datasheets and documents
References
ab102027 has not yet been referenced specifically in any publications.
