Recombinant Human Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 protein (Tagged) (ab235815)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Human Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 protein (Tagged)
See all Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
SEAEHRLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIM ETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDF QVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWS YDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDI TYSLYIRRL -
Predicted molecular weight
45 kDa including tags -
Amino acids
32 to 240 -
Tags
His tag N-Terminus -
Additional sequence information
Extracellular domain. N-terminal 10xHis-SUMO-tag and C-terminal Myc-tag.
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Associated products
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Related Products
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Myc tag antibody [Myc.A7] (ab18185)
- Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody (ab183097)
- Anti-Nicotinic Acetylcholine Receptor alpha 3/CHRNA3 antibody [6D3A10] (ab233717)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Myc tag antibody (ab9106)
- Anti-6X His tag® antibody (ab9108)
- Anti-Myc tag antibody - ChIP Grade (ab9132)
Specifications
Our Abpromise guarantee covers the use of ab235815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Additional notes
This product was previously labelled as Nicotinic Acetylcholine Receptor alpha 3
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- ACHA3_HUMAN
- AChR
- Cholinergic receptor neuronal nicotinic alpha polypeptide 3
see all -
Function
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-3/CHRNA3 sub-subfamily. -
Cellular localization
Cell junction > synapse > postsynaptic cell membrane. Cell membrane. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235815 has not yet been referenced specifically in any publications.