Recombinant Human NKF3 kinase family member/PEAK1 protein (ab164128)

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SDS-PAGE - Recombinant Human NKF3 kinase family member/PEAK1 protein (ab164128)

    Key features and details

    • Expression system: Wheat germ
    • Tags: GST tag N-Terminus
    • Suitable for: ELISA, WB

    Description

    • Product name

      Recombinant Human NKF3 kinase family member/PEAK1 protein
      See all NKF3 kinase family member/PEAK1 proteins and peptides

    • Expression system

      Wheat germ

    • Protein length

      Protein fragment

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Human

      • Sequence

        TPPPLPKKMIIRANTEPISKDLQKSMESSLCVMANPTYDIDPNWDASSAG SSISYELKGLDIESYDSLERPLRKERPVPSAANSISSLTTLSIKDRFSNS

      • Amino acids

        1151 to 1250

      • Tags

        GST tag N-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab164128 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      ELISA

      Western blot

    • Form

      Liquid

    • Additional notes

      This product was previously labelled as NKF3 kinase family member.

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.31% Glutathione, 0.79% Tris HCl

    General Info

    • Alternative names

      • FLJ21140
      • FLJ34483
      • KIAA2002
      • NKF3 kinase family member
      • PEAK1
      • PEAK1_HUMAN
      • pseudopodium enriched atypical kinase
      • Pseudopodium-enriched atypical kinase 1
      • SGK269
      • Sugen kinase 269
      • Tyrosine-protein kinase SgK269
      see all

    • Function

      Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.

    • Sequence similarities

      Belongs to the protein kinase superfamily.
      Contains 1 protein kinase domain.

    • Post-translational
      modifications

      Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro.

    • Cellular localization

      Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with F-actin in serum-rich medium. Actin colocalization is reduced during serum starvation.
    • Information by UniProt

    Images

    • SDS-PAGE - Recombinant Human NKF3 kinase family member/PEAK1 protein (ab164128)
      SDS-PAGE - Recombinant Human NKF3 kinase family member/PEAK1 protein (ab164128)
      ab164128 on a 12.5% SDS-PAGE stained with Coomassie Blue.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab164128? Please let us know so that we can cite the reference in this datasheet.

    ab164128 has not yet been referenced specifically in any publications.

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