Recombinant human NKG2D protein (Fc Chimera His Tag) (ab227395)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant human NKG2D protein (Fc Chimera His Tag)
See all NKG2D proteins and peptides -
Biological activity
Measured by its binding ability in a functional ELISA with Human ULBP-6/RAET1L. The ED50 range ≤ 1.5 ug/ml.
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Purity
> 95 % SDS-PAGE.
Affinity purified -
Endotoxin level
< 1.000 Eu/µg -
Expression system
Baculovirus infected insect cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ADPIWSAVFLNSLFNQEVQIPLTESYCGPCPKNWICYKNNCYQFFDESKN WYESQASCMSQNASLLKVYSKEDQDLLKLVKSYHWMGLVHIPTNGSWQWE DGSILSPNLLTIIEMQKGDCALYASSFKGYIENCSTPNTYICMQRTVVEP KSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVS HEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTC LVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRW QQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH -
Predicted molecular weight
44 kDa including tags -
Amino acids
73 to 216 -
Tags
His tag C-Terminus -
Additional sequence information
Extracellular domain fused to hIgG-His-tag at C-terminus (NP_031386).
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Associated products
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Related Buffer
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab227395 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 10% Glycerol, PBSThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- CD314
- CD314 antigen
- D12S2489E
see all -
Function
Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes. -
Tissue specificity
Natural killer cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Also detected in gamma-delta cells and CD8+ alpha-beta T-cells. Expressed in interferon-producing killer dendritic cells (IKDCs). -
Sequence similarities
Contains 1 C-type lectin domain. -
Cellular localization
Membrane. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227395 has not yet been referenced specifically in any publications.