Key features and details
- Expression system: Wheat germ
- Suitable for: WB, SDS-PAGE, ELISA
Product nameRecombinant Human Nkx2.5 protein
Expression systemWheat germ
Protein lengthFull length protein
SequenceMFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAA FKPEAYAGPEAAAPGLPELRAELGRAPSPAKCASAFPAAPAFYPRAYSDP DPAKDPRAEKKELCALQKAVELEKTEADNAERPRARRRRKPRVLFSQAQV YELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQ TLELVGLPPPPPPPARRIAVPVLVRDGKPCLGDSAPYAPAYGVGLNPYGY NAYPAYPGYGGAACSPGYSCTAAYPAGPSPAQPATAAANNNFVNFGVGDL NAVQSPGIPQSNSGVSTLHGIRAW
Predicted molecular weight61 kDa including tags
Amino acids1 to 324
Our Abpromise guarantee covers the use of ab152305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.31% Glutathione, 0.79% Tris HCl
- NKX2.5, mouse, homolog of
- Cardiac-specific homeobox
- Cardiac-specific homeobox 1
FunctionImplicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development.
Tissue specificityExpressed only in the heart.
Involvement in diseaseAtrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital
Sequence similaritiesBelongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab152305 has not yet been referenced specifically in any publications.