Recombinant human NME3 protein (ab211318)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, MS
Description
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Product name
Recombinant human NME3 protein
See all NME3 proteins and peptides -
Biological activity
Specific activity is > 150 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25°C in a couple system with PK/LDH.
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Purity
> 95 % SDS-PAGE.
ab211318 was purified using conventional chromatography. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MGSSHHHHHHSSGLVPRGSHMERTFLAVKPDGVQRRLVGEIVRRFERKGF KLVALKLVQASEELLREHYAELRERPFYGRLVKYMASGPVVAMVWQGLDV VRTSRALIGATNPADAPPGTIRGDFCIEVGKNLIHGSDSVESARREIALW FRADELLCWEDSAGHWLYE -
Predicted molecular weight
19 kDa including tags -
Amino acids
22 to 169 -
Tags
His tag N-Terminus -
Additional sequence information
NP_002504.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab211318 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Mass Spectrometry
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Mass spectrometry
MALDI-TOF -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.32% Tris HCl, 50% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.03% DTTThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- c371H6.2
- DR-nm23
- Expressed in non metastatic cells 3
see all -
Function
Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Probably has a role in normal hematopoiesis by inhibition of granulocyte differentiation and induction of apoptosis. -
Sequence similarities
Belongs to the NDK family. -
Developmental stage
Preferentially expressed at early stages of myeloid differentiation of highly purified CD34+ cells. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab211318 has not yet been referenced specifically in any publications.